ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFLFTFTILE SEKLIIWPVP AVVLGVMAPK RAYSTPFGPW PGPAECLWNC PSELRQFSSC 

        70         80         90        100        110        120 
CLPLPKLRPP RPTFASLWRV VAAIAALFQV PWRRKTGVGK AIFCIGCCCL AYQWRRTRYF 

       130 
AYKIMTGTCF MTDLAP 

References and documentation are available.

Number of amino acids: 136

Molecular weight: 15442.55

Theoretical pI: 9.59

Amino acid composition: 
Ala (A)  13	  9.6%
Arg (R)  10	  7.4%
Asn (N)   1	  0.7%
Asp (D)   1	  0.7%
Cys (C)   9	  6.6%
Gln (Q)   3	  2.2%
Glu (E)   4	  2.9%
Gly (G)   7	  5.1%
His (H)   0	  0.0%
Ile (I)   7	  5.1%
Leu (L)  13	  9.6%
Lys (K)   6	  4.4%
Met (M)   4	  2.9%
Phe (F)  10	  7.4%
Pro (P)  15	 11.0%
Ser (S)   6	  4.4%
Thr (T)   9	  6.6%
Trp (W)   6	  4.4%
Tyr (Y)   4	  2.9%
Val (V)   8	  5.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 5
Total number of positively charged residues (Arg + Lys): 16

Atomic composition:

Carbon      C	       720
Hydrogen    H	      1100
Nitrogen    N	       182
Oxygen      O	       170
Sulfur      S	        13

Formula: C720H1100N182O170S13
Total number of atoms: 2185

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    39460
Abs 0.1% (=1 g/l)   2.555, assuming all pairs of Cys residues form cystines


Ext. coefficient    38960
Abs 0.1% (=1 g/l)   2.523, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 62.04
This classifies the protein as unstable.



Aliphatic index: 83.97

Grand average of hydropathicity (GRAVY): 0.350