ProtParam
User-provided sequence:
10 20 30 40 50 60
MFLLSRFSDI ISIHPSNFWK PTKEALAEEI HKKYANKVIQ NIGLAICVYD FLKIGEGIIK
70 80 90 100 110 120
YGDGSSYMNV VFRLIIFRPF RGEVMLGKIK SCSEEGIRVT ISFFDDIFIP KDMLFDPCVF
130 140 150 160 170 180
RPDERAWVWK IEGEDGSEGT ELYFDIDEEI RFQIESEDFV DISPKRNKNA TAITGTEALE
190 200
SVSPYTLIAS CSRDGLGIPA WWK
References and
documentation are available.
Number of amino acids: 203
Molecular weight: 23242.69
Theoretical pI: 4.93
Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 1063
Hydrogen H 1628
Nitrogen N 264
Oxygen O 305
Sulfur S 8
Formula: C
1063H
1628N
264O
305S
8
Total number of atoms: 3268
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 36690
Abs 0.1% (=1 g/l) 1.579, assuming all pairs of Cys residues form cystines
Ext. coefficient 36440
Abs 0.1% (=1 g/l) 1.568, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 46.14
This classifies the protein as unstable.
Aliphatic index: 90.30
Grand average of hydropathicity (GRAVY): -0.090