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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFLRSVNRAV TRSILTTPKP AVVKSSWRVF TVANSKRCFT PAAIMRNQET QRVGDILQSE

70 80 90 100 110 120
LKIEKETLPE STSLDSFNDF LNKYKFSLVE TPGKNEAEIV RRTESGETVH VFFDVAQIAN

130 140 150 160 170 180
LPYNNAMDEN TEQNEDGINE DDFDALSDNF ANVNVVISKE SASEPAVSFE LLMNLQEGSF

190 200 210 220 230 240
YVDSATPYPS VDAALNQSAE AEITRELVYH GPPFSNLDEE LQESLEAYLE SRGVNEELAS

250 260
FISAYSEFKE NNEYISWLEK MKKFFH


References and documentation are available.

Number of amino acids: 266 Molecular weight: 30132.41 Theoretical pI: 4.52
Amino acid composition: 
Ala (A) 21 7.9% Arg (R) 11 4.1% Asn (N) 21 7.9% Asp (D) 13 4.9% Cys (C) 1 0.4% Gln (Q) 8 3.0% Glu (E) 32 12.0% Gly (G) 7 2.6% His (H) 3 1.1% Ile (I) 11 4.1% Leu (L) 21 7.9% Lys (K) 13 4.9% Met (M) 5 1.9% Phe (F) 17 6.4% Pro (P) 11 4.1% Ser (S) 27 10.2% Thr (T) 14 5.3% Trp (W) 2 0.8% Tyr (Y) 8 3.0% Val (V) 20 7.5% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 45 Total number of positively charged residues (Arg + Lys): 24 Atomic composition: Carbon C 1334 Hydrogen H 2053 Nitrogen N 349 Oxygen O 435 Sulfur S 6 Formula: C1334H2053N349O435S6 Total number of atoms: 4177 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 22920 Abs 0.1% (=1 g/l) 0.761, assuming all pairs of Cys residues form cystines Ext. coefficient 22920 Abs 0.1% (=1 g/l) 0.761, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 50.94 This classifies the protein as unstable. Aliphatic index: 76.62 Grand average of hydropathicity (GRAVY): -0.459