ProtParam
User-provided sequence:
10 20 30 40 50 60
MFLRSVNRAV TRSILTTPKP AVVKSSWRVF TVANSKRCFT PAAIMRNQET QRVGDILQSE
70 80 90 100 110 120
LKIEKETLPE STSLDSFNDF LNKYKFSLVE TPGKNEAEIV RRTESGETVH VFFDVAQIAN
130 140 150 160 170 180
LPYNNAMDEN TEQNEDGINE DDFDALSDNF ANVNVVISKE SASEPAVSFE LLMNLQEGSF
190 200 210 220 230 240
YVDSATPYPS VDAALNQSAE AEITRELVYH GPPFSNLDEE LQESLEAYLE SRGVNEELAS
250 260
FISAYSEFKE NNEYISWLEK MKKFFH
References and
documentation are available.
Number of amino acids: 266
Molecular weight: 30132.41
Theoretical pI: 4.52
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 1334
Hydrogen H 2053
Nitrogen N 349
Oxygen O 435
Sulfur S 6
Formula: C
1334H
2053N
349O
435S
6
Total number of atoms: 4177
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.761, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.761, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 50.94
This classifies the protein as unstable.
Aliphatic index: 76.62
Grand average of hydropathicity (GRAVY): -0.459