ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFLTRSEYDR GVSTFSPEGR LFQVEYSLEA IKLGSTAIGI ATKEGVVLGV EKRATSPLLE 

        70         80         90        100        110        120 
SDSIEKIVEI DRHIGCAMSG LTADARSMIE HARTAAVTHN LYYDEDINVE SLTQSVCDLA 

       130        140        150        160        170        180 
LRFGEGASGE ERLMSRPFGV ALLIAGHDAD DGYQLFHAEP SGTFYRYNAK AIGSGSEGAQ 

       190        200        210        220        230        240 
AELLNEWHSS LTLKEAELLV LKILKQVMEE KLDENNAQLS CITKQDGFKI YDNEKTAELI 

       250        260 
KELKEKEAAE SPEEADVEMS 

References and documentation are available.

Number of amino acids: 260

Molecular weight: 28617.12

Theoretical pI: 4.67

Amino acid composition: 
Ala (A)  26	 10.0%
Arg (R)  11	  4.2%
Asn (N)   7	  2.7%
Asp (D)  14	  5.4%
Cys (C)   3	  1.2%
Gln (Q)   7	  2.7%
Glu (E)  33	 12.7%
Gly (G)  19	  7.3%
His (H)   6	  2.3%
Ile (I)  15	  5.8%
Leu (L)  28	 10.8%
Lys (K)  15	  5.8%
Met (M)   6	  2.3%
Phe (F)   8	  3.1%
Pro (P)   5	  1.9%
Ser (S)  22	  8.5%
Thr (T)  13	  5.0%
Trp (W)   1	  0.4%
Tyr (Y)   8	  3.1%
Val (V)  13	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 26

Atomic composition:

Carbon      C	      1252
Hydrogen    H	      1991
Nitrogen    N	       335
Oxygen      O	       412
Sulfur      S	         9

Formula: C1252H1991N335O412S9
Total number of atoms: 3999

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    17545
Abs 0.1% (=1 g/l)   0.613, assuming all pairs of Cys residues form cystines


Ext. coefficient    17420
Abs 0.1% (=1 g/l)   0.609, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 49.38
This classifies the protein as unstable.



Aliphatic index: 89.00

Grand average of hydropathicity (GRAVY): -0.299