ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFNLLNTYIT SPLDQFEIRT LFGLQSSFID LSCLNLTTFS LYTIIVLLVI TSLYTLTNNN 

        70         80         90        100        110        120 
NKIIGSRWLI SQEAIYDTIM NMTKGQIGGK NWGLYFPMIF TLFMFIFIAN LISMIPYSFA 

       130        140        150        160        170        180 
LSAHLVFIIS LSIVIWLGNT ILGLYKHGWV FFSLFVPAGT PLPLVPLLVI IETLSYFARA 

       190        200        210        220        230        240 
ISLGLRLGSN ILAGHLLMVI LAGLTFNFML INLFTLVFGF VPLAMILAIM MLEFAIGIIQ 

       250 
GYVWAILTAS YLKDAVYLH 

References and documentation are available.

Number of amino acids: 259

Molecular weight: 29099.04

Theoretical pI: 7.85

Amino acid composition: 
Ala (A)  15	  5.8%
Arg (R)   4	  1.5%
Asn (N)  14	  5.4%
Asp (D)   4	  1.5%
Cys (C)   1	  0.4%
Gln (Q)   5	  1.9%
Glu (E)   4	  1.5%
Gly (G)  17	  6.6%
His (H)   4	  1.5%
Ile (I)  34	 13.1%
Leu (L)  46	 17.8%
Lys (K)   5	  1.9%
Met (M)  11	  4.2%
Phe (F)  22	  8.5%
Pro (P)   8	  3.1%
Ser (S)  18	  6.9%
Thr (T)  18	  6.9%
Trp (W)   5	  1.9%
Tyr (Y)  11	  4.2%
Val (V)  13	  5.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 8
Total number of positively charged residues (Arg + Lys): 9

Atomic composition:

Carbon      C	      1395
Hydrogen    H	      2156
Nitrogen    N	       308
Oxygen      O	       342
Sulfur      S	        12

Formula: C1395H2156N308O342S12
Total number of atoms: 4213

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    43890
Abs 0.1% (=1 g/l)   1.508, assuming all pairs of Cys residues form cystines


Ext. coefficient    43890
Abs 0.1% (=1 g/l)   1.508, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.57
This classifies the protein as stable.



Aliphatic index: 140.81

Grand average of hydropathicity (GRAVY): 1.097