ProtParam
User-provided sequence:
10 20 30 40 50 60
MFNNQLPLVD LHRHLDGNIR PKTIWQLAQQ NNIKLPEDNF EAFIPHVQIT DSEADLLAFL
70 80 90 100 110 120
KKLDWGVGVL KSLDDVVRIG FENVEDAYNA NIDYAELRFS PYYIAMTHNL PIEGVVEAII
130 140 150 160 170 180
EGVNQGRKKF TTKINLMGIL SRTFGVEHCQ SELNALLAYK DDLVAVDLAG DEYNFPGSLF
190 200 210 220 230 240
ESHFKQVNDA GLNVSVHAGE AAGPESVWHA IKTLGATRIG HGVACAKDQE LMDYMRENKI
250 260 270 280 290 300
SIESCLTSNY QTGTIKDLAV HPVKTFLAND LTVCLNTDDP AVENIELAGE FQVAREVLGL
310 320 330
NTDQITQLQR NAVQMSFLSE QEKTALLNIK Q
References and
documentation are available.
Number of amino acids: 331
Molecular weight: 36883.78
Theoretical pI: 4.86
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 27
Atomic composition:
Carbon C 1641
Hydrogen H 2578
Nitrogen N 442
Oxygen O 504
Sulfur S 10
Formula: C
1641H
2578N
442O
504S
10
Total number of atoms: 5175
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 28670
Abs 0.1% (=1 g/l) 0.777, assuming all pairs of Cys residues form cystines
Ext. coefficient 28420
Abs 0.1% (=1 g/l) 0.771, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 41.78
This classifies the protein as unstable.
Aliphatic index: 98.70
Grand average of hydropathicity (GRAVY): -0.172