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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFNNQLPLVD LHRHLDGNIR PKTIWQLAQQ NNIKLPEDNF EAFIPHVQIT DSEADLLAFL

70 80 90 100 110 120
KKLDWGVGVL KSLDDVVRIG FENVEDAYNA NIDYAELRFS PYYIAMTHNL PIEGVVEAII

130 140 150 160 170 180
EGVNQGRKKF TTKINLMGIL SRTFGVEHCQ SELNALLAYK DDLVAVDLAG DEYNFPGSLF

190 200 210 220 230 240
ESHFKQVNDA GLNVSVHAGE AAGPESVWHA IKTLGATRIG HGVACAKDQE LMDYMRENKI

250 260 270 280 290 300
SIESCLTSNY QTGTIKDLAV HPVKTFLAND LTVCLNTDDP AVENIELAGE FQVAREVLGL

310 320 330
NTDQITQLQR NAVQMSFLSE QEKTALLNIK Q


References and documentation are available.

Number of amino acids: 331 Molecular weight: 36883.78 Theoretical pI: 4.86
Amino acid composition: 
Ala (A) 28 8.5% Arg (R) 10 3.0% Asn (N) 24 7.3% Asp (D) 22 6.6% Cys (C) 4 1.2% Gln (Q) 17 5.1% Glu (E) 24 7.3% Gly (G) 20 6.0% His (H) 10 3.0% Ile (I) 21 6.3% Leu (L) 37 11.2% Lys (K) 17 5.1% Met (M) 6 1.8% Phe (F) 14 4.2% Pro (P) 10 3.0% Ser (S) 14 4.2% Thr (T) 17 5.1% Trp (W) 3 0.9% Tyr (Y) 8 2.4% Val (V) 25 7.6% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 46 Total number of positively charged residues (Arg + Lys): 27 Atomic composition: Carbon C 1641 Hydrogen H 2578 Nitrogen N 442 Oxygen O 504 Sulfur S 10 Formula: C1641H2578N442O504S10 Total number of atoms: 5175 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 28670 Abs 0.1% (=1 g/l) 0.777, assuming all pairs of Cys residues form cystines Ext. coefficient 28420 Abs 0.1% (=1 g/l) 0.771, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 41.78 This classifies the protein as unstable. Aliphatic index: 98.70 Grand average of hydropathicity (GRAVY): -0.172