ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFNRFNKLQA ALALVLYSQS ALGQYYTNSS SIASNSSTAV SSTSSGSVSI SSSIELTSST 

        70         80         90        100        110        120 
SDVSSSLTEL TSSSTEVSSS IAPSTSSSEV SSSITSSGSS VSGSSSITSS GSSVSSSSSA 

       130        140        150        160        170        180 
TESGSSASGS SSATESGSSV SGSSTSITSG SSSATESGSS VSGSTSATES GSSASGSSSA 

       190        200        210        220        230        240 
TESGSSASGS SSATESGSSV SGSSSATESG SSVSGSSSAT ESGSASSVPS SSGSVTESGS 

       250        260        270        280        290        300 
SSSASESSIT QSGTASGSSA SSTSGSVTQS GSSVSGSSAS SAPGISSSIP QSTSSASTAS 

       310        320        330        340        350        360 
GSITSGTLSS ITSSASSATA TASNSLSSSD GTIYLPSTTI SGDITLTGSV IATEAVEVAA 

       370        380        390        400        410        420 
GGKLTLLDGD KYVFSADFII HGGVFVEKSK PTYPGTEFDI SGENFDVSGT FNAEEPAASS 

       430        440        450        460        470        480 
ASAYSFTPGS FDNSGDISLS LSESTKGEVT FSPYSNSGAF SFSNAILNGG SVSGLQRRAE 

       490        500        510        520        530        540 
SGSVNNGEIN LENGSTYVVV EPVSGSGTIN IISGNLYLHY PDTFTGQTVV FKGEGVLAVD 

       550        560        570        580        590        600 
PTETNTTPIP VVGYTGENQI AITADVTALS YDSATGVLTA TQGNSQFSFS IGTGFSSSGF 

       610        620        630        640        650        660 
NVSEGTFAGA YAYYLNYGGV VASSATPSST STTSGATNST SGSTSFGASV TGSTASTSFG 

       670        680        690        700        710        720 
ASVTGSTAST LISGSPSVYT TTLTYATTTS TVVVSCSETT DSNGNVYTIT TTVPCSSTTA 

       730        740        750        760        770        780 
TITSCDETGC HVTTSTGTVA TETVSSKSYT TVTVTHCDNN GCNTKTVTSE CPEETSATTT 

       790        800        810        820        830        840 
SPKSYTTVTV THCDDNGCNT KTVTSEAPEA TTTTVSPKTY TTATVTQCDD NGCSTKTVTS 

       850        860        870        880        890        900 
EAPKETSETS ETSAAPKTYT TATVTQCDDN GCNVKIITSQ IPEATSTVTA TSASPKSYTT 

       910        920        930        940        950        960 
VTSEGSKATS LTTAISKASS AISTYSKSAA PIKTSTGIIV QSEGIAAGLN ANTLNALVGI 


FVLAFFN 

References and documentation are available.

Number of amino acids: 967

Molecular weight: 94705.62

Theoretical pI: 4.18

Amino acid composition: 
Ala (A)  84	  8.7%
Arg (R)   3	  0.3%
Asn (N)  37	  3.8%
Asp (D)  23	  2.4%
Cys (C)  13	  1.3%
Gln (Q)  15	  1.6%
Glu (E)  46	  4.8%
Gly (G)  88	  9.1%
His (H)   5	  0.5%
Ile (I)  42	  4.3%
Leu (L)  33	  3.4%
Lys (K)  21	  2.2%
Met (M)   1	  0.1%
Phe (F)  25	  2.6%
Pro (P)  27	  2.8%
Ser (S) 255	 26.4%
Thr (T) 155	 16.0%
Trp (W)   0	  0.0%
Tyr (Y)  26	  2.7%
Val (V)  68	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 69
Total number of positively charged residues (Arg + Lys): 24

Atomic composition:

Carbon      C	      3960
Hydrogen    H	      6307
Nitrogen    N	      1059
Oxygen      O	      1594
Sulfur      S	        14

Formula: C3960H6307N1059O1594S14
Total number of atoms: 12934

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    39490
Abs 0.1% (=1 g/l)   0.417, assuming all pairs of Cys residues form cystines


Ext. coefficient    38740
Abs 0.1% (=1 g/l)   0.409, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.39
This classifies the protein as unstable.



Aliphatic index: 59.33

Grand average of hydropathicity (GRAVY): -0.108