ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFNRTTQLKS KHPCSVCTRR KVKCDRMIPC GNCRKRGQDS ECMKSTKLIT ASSSKEYLPD 

        70         80         90        100        110        120 
LLLFWQNYEY WITNIGLYKT KQRDLTRTPA NLDTDTEECM FWMNYLQKDQ SFQLMNFAME 

       130        140        150        160        170        180 
NLGALYFGSI GDISELYLRV EQYWDRRADK NHSVDGKYWD ALIWSVFTMC IYYMPVEKLA 

       190        200        210        220        230        240 
EIFSVYPLHE YLGSNKRLNW EDGMQLVMCQ NFARCSLFQL KQCDFMAHPD IRLVQAYLIL 

       250        260        270        280        290        300 
ATTTFPYDEP LLANSLLTQC IHTFKNFHVD DFRPLLNDDP VESIAKVTLG RIFYRLCGCD 

       310        320        330        340        350        360 
YLQSGPRKPI ALHTEVSSLL QHAAYLQDLP NVDVYREENS TEVLYWKIIS LDRDLDQYLN 

       370        380        390        400        410        420 
KSSKPPLKTL DAIRRELDIF QYKVDSLEED FRSNNSRFQK FIALFQISTV SWKLFKMYLI 

       430        440        450        460        470        480 
YYDTADSLLK VIHYSKVIIS LIVNNFHAKS EFFNRHPMVM QTITRVVSFI SFYQIFVESA 

       490        500        510        520        530        540 
AVKQLLVDLT ELTANLPTIF GSKLDKLVYL TERLSKLKLL WDKVQLLDSG DSFYHPVFKI 

       550        560        570        580        590        600 
LQNDIKIIEL KNDEMFSLIK GLGSLVPLNK LRQESLLEEE DENNTEPSDF RTIVEEFQSE 


YNISDILS 

References and documentation are available.

Number of amino acids: 608

Molecular weight: 71358.14

Theoretical pI: 5.93

Amino acid composition: 
Ala (A)  23	  3.8%
Arg (R)  29	  4.8%
Asn (N)  30	  4.9%
Asp (D)  42	  6.9%
Cys (C)  14	  2.3%
Gln (Q)  28	  4.6%
Glu (E)  36	  5.9%
Gly (G)  16	  2.6%
His (H)  12	  2.0%
Ile (I)  38	  6.2%
Leu (L)  78	 12.8%
Lys (K)  41	  6.7%
Met (M)  16	  2.6%
Phe (F)  34	  5.6%
Pro (P)  21	  3.5%
Ser (S)  47	  7.7%
Thr (T)  31	  5.1%
Trp (W)  10	  1.6%
Tyr (Y)  30	  4.9%
Val (V)  32	  5.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 78
Total number of positively charged residues (Arg + Lys): 70

Atomic composition:

Carbon      C	      3235
Hydrogen    H	      5008
Nitrogen    N	       828
Oxygen      O	       931
Sulfur      S	        30

Formula: C3235H5008N828O931S30
Total number of atoms: 10032

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient   100575
Abs 0.1% (=1 g/l)   1.409, assuming all pairs of Cys residues form cystines


Ext. coefficient    99700
Abs 0.1% (=1 g/l)   1.397, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 40.57
This classifies the protein as unstable.



Aliphatic index: 93.45

Grand average of hydropathicity (GRAVY): -0.244