ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFNRVFTRSF ASSLRAAASK AAAPPPVRLF GVEGTYATAL YQAAAKNSSI DAAFQSLQKV 

        70         80         90        100        110        120 
ESTVKKNPKL GHLLLNPALS LKDRNSVIDA IVETHKNLDG YVVNLLKVLS ENNRLGCFEK 

       130        140        150        160        170        180 
IASDFGVLND AHNGLLKGTV TSAEPLDPKS FKRIEKALSA SKLVGQGKSL KLENVVKPEI 

       190        200        210 
KGGLIVELGD KTVDLSISTK IQKLNKVLED SI 

References and documentation are available.

Number of amino acids: 212

Molecular weight: 22814.40

Theoretical pI: 9.58

Amino acid composition: 
Ala (A)  21	  9.9%
Arg (R)   7	  3.3%
Asn (N)  13	  6.1%
Asp (D)  10	  4.7%
Cys (C)   1	  0.5%
Gln (Q)   5	  2.4%
Glu (E)  11	  5.2%
Gly (G)  13	  6.1%
His (H)   3	  1.4%
Ile (I)  10	  4.7%
Leu (L)  28	 13.2%
Lys (K)  23	 10.8%
Met (M)   1	  0.5%
Phe (F)   8	  3.8%
Pro (P)   8	  3.8%
Ser (S)  20	  9.4%
Thr (T)   9	  4.2%
Trp (W)   0	  0.0%
Tyr (Y)   3	  1.4%
Val (V)  18	  8.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 21
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      1020
Hydrogen    H	      1684
Nitrogen    N	       280
Oxygen      O	       305
Sulfur      S	         2

Formula: C1020H1684N280O305S2
Total number of atoms: 3291

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient     4470
Abs 0.1% (=1 g/l)   0.196, assuming all pairs of Cys residues form cystines


Ext. coefficient     4470
Abs 0.1% (=1 g/l)   0.196, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 24.27
This classifies the protein as stable.



Aliphatic index: 104.43

Grand average of hydropathicity (GRAVY): -0.094