ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFNTTPINID KWLKENEGLL KPPVNNYCLH KGGFTVMIVG GPNERTGYHI NPTPEWFYQK 

        70         80         90        100        110        120 
KGSMLLKVVD ETDAEPKFID IIINEGDSYL LPGNVPHSPV RFADTVGIVV EQDRPGGEND 

       130        140        150        160        170 
KIRWYCSHCR QVVHESELQM LDLGTQVKEA ILDFENDVEK RTCFHCKTLN YARPQSN 

References and documentation are available.

Number of amino acids: 177

Molecular weight: 20234.98

Theoretical pI: 5.50

Amino acid composition: 
Ala (A)   4	  2.3%
Arg (R)   7	  4.0%
Asn (N)  13	  7.3%
Asp (D)  11	  6.2%
Cys (C)   5	  2.8%
Gln (Q)   6	  3.4%
Glu (E)  14	  7.9%
Gly (G)  13	  7.3%
His (H)   6	  3.4%
Ile (I)  11	  6.2%
Leu (L)  13	  7.3%
Lys (K)  12	  6.8%
Met (M)   4	  2.3%
Phe (F)   7	  4.0%
Pro (P)  12	  6.8%
Ser (S)   6	  3.4%
Thr (T)  10	  5.6%
Trp (W)   3	  1.7%
Tyr (Y)   6	  3.4%
Val (V)  14	  7.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 19

Atomic composition:

Carbon      C	       901
Hydrogen    H	      1392
Nitrogen    N	       244
Oxygen      O	       269
Sulfur      S	         9

Formula: C901H1392N244O269S9
Total number of atoms: 2815

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    25690
Abs 0.1% (=1 g/l)   1.270, assuming all pairs of Cys residues form cystines


Ext. coefficient    25440
Abs 0.1% (=1 g/l)   1.257, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 38.83
This classifies the protein as stable.



Aliphatic index: 78.08

Grand average of hydropathicity (GRAVY): -0.529