ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFPLNFHYED VSRQDPLLKP NHANVMEVPG SCEIRVVPKA PYNFIIKNGK LAMEIPRGQK 

        70         80         90        100        110        120 
FIQTQRGSTG KSFRSNPFLG SNKDKGYVSD LARQSTLRGH GMSNFSVRIS TVMSLLDFPV 

       130        140        150        160        170        180 
EIRKNSIQFS METEFCEFSP ELEDHFEIFE HIRGFNVTII TSANTQDETL PLWSGFLQKD 


EGETQ 

References and documentation are available.

Number of amino acids: 185

Molecular weight: 21109.91

Theoretical pI: 5.97

Amino acid composition: 
Ala (A)   5	  2.7%
Arg (R)  10	  5.4%
Asn (N)  11	  5.9%
Asp (D)   8	  4.3%
Cys (C)   2	  1.1%
Gln (Q)   9	  4.9%
Glu (E)  15	  8.1%
Gly (G)  12	  6.5%
His (H)   5	  2.7%
Ile (I)  12	  6.5%
Leu (L)  13	  7.0%
Lys (K)  10	  5.4%
Met (M)   6	  3.2%
Phe (F)  15	  8.1%
Pro (P)  11	  5.9%
Ser (S)  17	  9.2%
Thr (T)  10	  5.4%
Trp (W)   1	  0.5%
Tyr (Y)   3	  1.6%
Val (V)  10	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 23
Total number of positively charged residues (Arg + Lys): 20

Atomic composition:

Carbon      C	       940
Hydrogen    H	      1454
Nitrogen    N	       256
Oxygen      O	       282
Sulfur      S	         8

Formula: C940H1454N256O282S8
Total number of atoms: 2940

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    10095
Abs 0.1% (=1 g/l)   0.478, assuming all pairs of Cys residues form cystines


Ext. coefficient     9970
Abs 0.1% (=1 g/l)   0.472, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.82
This classifies the protein as stable.



Aliphatic index: 71.08

Grand average of hydropathicity (GRAVY): -0.462