ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFPPPRKCTR FRPLSLQLPK PLFPIAGVPL IEHHIDQLCQ LSGLSEILLL GFFPSDVFTD 

        70         80         90        100        110        120 
FISRCQQTYR VSIKYLEEPN PLGTAGGLVS FKKQILAGDP DAVFVINADV CGDLPIEDMG 

       130        140        150        160        170        180 
AKLDSLSGSS MLMLTTEATR QQSINFGSVV TDSEGRVIHY VDKPTTFVST NISCGVYLIK 

       190        200        210        220        230        240 
AEVIRQLDLP LNGDGIWLET DVLPQLASSG NLYALHTTRW WSQTKTAAAV LYANRHYLRL 

       250        260        270        280        290        300 
YKRRYAARLC KNGAQIIGDV FIDPSAKVHP TAKIGPNVSI GPKSVIGKGV RIKESIILPE 

       310        320        330        340        350        360 
AVIEENACVL QSVIGWRSVV GMWARIEGIP LEPNPNLPFA KMDNKPLFLP DGRLTPSLTI 

       370        380        390 
LGSDVSVAPE TIILNCVVLP YKELTCSYKN QIIL 

References and documentation are available.

Number of amino acids: 394

Molecular weight: 43326.47

Theoretical pI: 8.01

Amino acid composition: 
Ala (A)  24	  6.1%
Arg (R)  18	  4.6%
Asn (N)  15	  3.8%
Asp (D)  19	  4.8%
Cys (C)   9	  2.3%
Gln (Q)  14	  3.6%
Glu (E)  17	  4.3%
Gly (G)  27	  6.9%
His (H)   6	  1.5%
Ile (I)  33	  8.4%
Leu (L)  46	 11.7%
Lys (K)  20	  5.1%
Met (M)   6	  1.5%
Phe (F)  14	  3.6%
Pro (P)  30	  7.6%
Ser (S)  29	  7.4%
Thr (T)  21	  5.3%
Trp (W)   5	  1.3%
Tyr (Y)  11	  2.8%
Val (V)  30	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 38

Atomic composition:

Carbon      C	      1963
Hydrogen    H	      3132
Nitrogen    N	       514
Oxygen      O	       557
Sulfur      S	        15

Formula: C1963H3132N514O557S15
Total number of atoms: 6181

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44390
Abs 0.1% (=1 g/l)   1.025, assuming all pairs of Cys residues form cystines


Ext. coefficient    43890
Abs 0.1% (=1 g/l)   1.013, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.91
This classifies the protein as unstable.



Aliphatic index: 106.37

Grand average of hydropathicity (GRAVY): 0.113