ProtParam
User-provided sequence:
10 20 30 40 50 60
MFPSSLSALR FFLGFVNFNF SALINAHYTV SSYRPQPRST RRRMTKKSPK ANGGFFPALM
70 80 90 100 110 120
LVGLIGAGIW YFSPSGGSKN LPPVNLGAIA DNLPQGINPK TLVVTQRVDN PIAGNTKSFS
130 140 150 160 170 180
VDGGERLDNL ARTINYQGTS VKELADLIRP LASNDWEEAR LAYSWITQNI AYDVPMAETR
190 200 210 220 230 240
NIDDLRPETV LARGETICSG YSNLYQALAK ELGLDVVIIE GFAKGGDVIV GDDPDVNHAW
250 260 270 280 290 300
NGVKIDGQWY LLDTTWGAGI VSDGKFEAKF NPTYFATAPE QLIVSHFPRE SQWQLLPQPY
310 320 330 340 350 360
DRQTFDQLPA LTPRFFRDQI ALVSHPKNDI VAGGDLEVIL RTGNDVQVVA QLMDNNGQPL
370 380 390 400 410 420
DKQYTLSQSQ QSQVTVNAAF PQPGEYQLLI LSKNSQEDTY YQAIAYDVKA QGAGQAFPST
430 440 450 460 470 480
YGTFSEKQVR LISPLGRNLP QNQASYFQLQ VPGAEKVVLV DEQRQELIPL DRTGDVFTGS
490 500
EIIRFDNVTV AAQFPGSNQF WSLLEYE
References and
documentation are available.
Number of amino acids: 507
Molecular weight: 56178.23
Theoretical pI: 5.04
Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 42
Atomic composition:
Carbon C 2530
Hydrogen H 3897
Nitrogen N 679
Oxygen O 760
Sulfur S 6
Formula: C
2530H
3897N
679O
760S
6
Total number of atoms: 7872
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 72310
Abs 0.1% (=1 g/l) 1.287, assuming all pairs of Cys residues form cystines
Ext. coefficient 72310
Abs 0.1% (=1 g/l) 1.287, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.12
This classifies the protein as stable.
Aliphatic index: 86.17
Grand average of hydropathicity (GRAVY): -0.277