ProtParam
User-provided sequence:
10 20 30 40 50 60
MFPYLTRMNL SIKMGGLTLK ESSPNAFLNN TTIARRFKHE YAPRFKIVQK KQKGRVPVRT
70 80 90 100 110 120
GGSIKGSTLQ FGKYGLRLKS EGIRISAQQL KEADNAIMRY VRPLNNGHLW RRLCTNVAVC
130 140 150 160 170 180
IKGNETRMGK GKGGFDHWMV RVPTGKILFE INGDDLHEKV AREAFRKAGT KLPGVYEFVS
190 200 210 220 230
LDSLVRVGLH SFKNPKDDPV KNFYDENAKK PSKKYLNILK SQEPQYKLFR GR
References and
documentation are available.
Number of amino acids: 232
Molecular weight: 26517.91
Theoretical pI: 10.32
Total number of negatively charged residues (Asp + Glu): 19
Total number of positively charged residues (Arg + Lys): 46
Atomic composition:
Carbon C 1190
Hydrogen H 1913
Nitrogen N 349
Oxygen O 322
Sulfur S 8
Formula: C
1190H
1913N
349O
322S
8
Total number of atoms: 3782
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23045
Abs 0.1% (=1 g/l) 0.869, assuming all pairs of Cys residues form cystines
Ext. coefficient 22920
Abs 0.1% (=1 g/l) 0.864, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.58
This classifies the protein as stable.
Aliphatic index: 76.03
Grand average of hydropathicity (GRAVY): -0.619