ProtParam
User-provided sequence:
10 20 30 40 50 60
MFQFVTPVGT QNGLKATCAK ISPDGQFLAI TQGLNILIYD INRRTVSQTL VTSHARPFSE
70 80 90 100 110 120
LCWSPDGQCI ATASDDFSVE IIHLSYGLLH TFIGHTAPVI SLTFNRKGNL LFTSSMDESI
130 140 150 160 170 180
KIWDTLNGSL MKTISAHSEA VVSVDVPMND SSILSSGSYD GLIRIFDAET GHCLKTLTYD
190 200 210 220 230 240
KDWKRENGVV PISQVKFSEN ARYLLVKSLD GVVKIWDCIG GCVVRTFQVQ PLEKGVLHHS
250 260 270 280 290 300
CGMDFLNPED GSTPLVISGY ENGDIYCWNS DTKSLLQLLD GSLYHHSSPV MSIHCFGNIM
310
CSLALNGDCC LWRWV
References and
documentation are available.
Number of amino acids: 315
Molecular weight: 34753.72
Theoretical pI: 5.60
Total number of negatively charged residues (Asp + Glu): 31
Total number of positively charged residues (Arg + Lys): 22
Atomic composition:
Carbon C 1547
Hydrogen H 2406
Nitrogen N 408
Oxygen O 464
Sulfur S 19
Formula: C
1547H
2406N
408O
464S
19
Total number of atoms: 4844
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 51170
Abs 0.1% (=1 g/l) 1.472, assuming all pairs of Cys residues form cystines
Ext. coefficient 50420
Abs 0.1% (=1 g/l) 1.451, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 35.92
This classifies the protein as stable.
Aliphatic index: 94.32
Grand average of hydropathicity (GRAVY): 0.072