ProtParam
User-provided sequence:
10 20 30 40 50 60
MFRDRTNLFL SYRRTFPHNI TFSSGKAPLG DDQDIEMGTY PMMNMSHDIS ARLTDERKNK
70 80 90 100 110 120
HENHSDALPP IFIDIAQDVD DYLLEVRRLS EQLAKVYRKN SLPGFEDKSH DEALIEDLSF
130 140 150 160 170 180
KVIQMLQKCY AVMKRLKTIY NSQFVDGKQL SREELIILDN LQKIYAEKIQ TESNKFRVLQ
190 200 210 220 230 240
NNYLKFLNKD DLKPIRNKAS AENTLLLDDE EEEAAREKRE GLDIEDYSKR TLQRQQQLHD
250 260 270 280 290 300
TSAEAYLRER DEEITQLARG VLEVSTIFRE MQDLVVDQGT IVDRIDYNLE NTVVELKSAD
310 320 330 340 350 360
KELNKATHYQ KRTQKCKVIL LLTLCVIALF FFVMLKPHGG GSGGRNNGSN KYNNDDNKTV
370 380 390
NNSHDDGSNT HINDEESNLP SIVEVTESEN DALDDLL
References and
documentation are available.
Number of amino acids: 397
Molecular weight: 45875.46
Theoretical pI: 5.10
Total number of negatively charged residues (Asp + Glu): 71
Total number of positively charged residues (Arg + Lys): 53
Atomic composition:
Carbon C 1999
Hydrogen H 3192
Nitrogen N 566
Oxygen O 646
Sulfur S 12
Formula: C
1999H
3192N
566O
646S
12
Total number of atoms: 6415
Extinction coefficients:
This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 19495
Abs 0.1% (=1 g/l) 0.425, assuming all pairs of Cys residues form cystines
Ext. coefficient 19370
Abs 0.1% (=1 g/l) 0.422, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 44.76
This classifies the protein as unstable.
Aliphatic index: 86.20
Grand average of hydropathicity (GRAVY): -0.716