ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFRQSKRRIA SRKNFSSYDD IVKSELDVGN TNAANQIILS SSSSEEEKKL YARLYESKLS 

        70         80         90        100        110        120 
FYDLPPQGEI TLEQFEIWAI DRLKILLEIE SCLSRNKSIK EIETIIKPQF QKLLPFNTES 

       130        140        150        160        170        180 
LEDRKKDYYS HFILRLCFCR SKELREKFVR AETFLFKIRF NMLTSTDQTK FVQSLDLPLL 

       190        200        210        220        230        240 
QFISNEEKAE LSHQLYQTVS ASLQFQLNLN EEHQRKQYFQ QEKFIKLPFE NVIELVGNRL 

       250        260        270        280        290        300 
VFLKDGYAYL PQFQQLNLLS NEFASKLNQE LIKTYQYLPR LNEDDRLLPI LNHLSSGYTI 

       310        320        330        340        350        360 
ADFNQQKANQ FSENVDDEIN AQSVWSEEIS SNYPLCIKNL MEGLKKNHHL RYYGRQQLSL 

       370        380        390        400        410        420 
FLKGIGLSAD EALKFWSEAF TRNGNMTMEK FNKEYRYSFR HNYGLEGNRI NYKPWDCHTI 

       430        440        450        460        470        480 
LSKPRPGRGD YHGCPFRDWS HERLSAELRS MKLTQAQIIS VLDSCQKGEY TIACTKVFEM 

       490        500        510        520 
THNSASADLE IGEQTHIAHP NLYFERSRQL QKKQQKLEKE KLFNNGNH 

References and documentation are available.

Number of amino acids: 528

Molecular weight: 62262.68

Theoretical pI: 8.38

Amino acid composition: 
Ala (A)  22	  4.2%
Arg (R)  30	  5.7%
Asn (N)  35	  6.6%
Asp (D)  21	  4.0%
Cys (C)   8	  1.5%
Gln (Q)  36	  6.8%
Glu (E)  47	  8.9%
Gly (G)  18	  3.4%
His (H)  14	  2.7%
Ile (I)  32	  6.1%
Leu (L)  64	 12.1%
Lys (K)  42	  8.0%
Met (M)   7	  1.3%
Phe (F)  32	  6.1%
Pro (P)  15	  2.8%
Ser (S)  46	  8.7%
Thr (T)  19	  3.6%
Trp (W)   5	  0.9%
Tyr (Y)  23	  4.4%
Val (V)  12	  2.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 68
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      2791
Hydrogen    H	      4340
Nitrogen    N	       764
Oxygen      O	       824
Sulfur      S	        15

Formula: C2791H4340N764O824S15
Total number of atoms: 8734

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    62270
Abs 0.1% (=1 g/l)   1.000, assuming all pairs of Cys residues form cystines


Ext. coefficient    61770
Abs 0.1% (=1 g/l)   0.992, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 45.15
This classifies the protein as unstable.



Aliphatic index: 81.67

Grand average of hydropathicity (GRAVY): -0.655