ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFRRGDNSNF NVQNSFFLPL EYEYTVKDNV PSKKKSSIGF FPLDDSLFTS KNNSGHHKSE 

        70         80         90        100        110        120 
QLHRGNAETI RSQFGTDAVP IRIDEKEGKW DRIQDDNSSN LNYQINNSND PASSGKYTQS 

       130        140        150        160        170        180 
IDCNHIAESK FSKKNGNIDS LRSNSATFML NTADEDVIEF SFDDNVPYAE LLSGATLEKC 

       190        200        210        220        230        240 
SLTLNEINKK LFNTLYDFRV SKDNPEENLV ELILPNCVVL LNFFEDIELL ADSSDEAFEK 

       250        260        270        280        290        300 
STFINTIEFI VHDIWVETLI KNINLLQMFD ADLKCYNDKY IICKLKGQYP STNIVDIMCR 

       310        320        330        340        350        360 
LKHFSNSILE TFKFGIELKE QDQCHNRNTI INYVLFSRVF STIVLEIQKC FILIVKFMYS 

       370        380        390        400        410        420 
VNFLEKFSDE VFLSFIEILI KIVFEHQIPQ LFLGIDEIIQ LWLKNNEGKR QQLLSAWCNG 

       430        440        450        460        470 
TVQDMKQSQQ RESSNAESES ITSSTEEDEE GLQFNKWDVI EPFIDNIKAL NQSKSHM 

References and documentation are available.

Number of amino acids: 477

Molecular weight: 55138.12

Theoretical pI: 4.85

Amino acid composition: 
Ala (A)  14	  2.9%
Arg (R)  13	  2.7%
Asn (N)  44	  9.2%
Asp (D)  33	  6.9%
Cys (C)   9	  1.9%
Gln (Q)  22	  4.6%
Glu (E)  39	  8.2%
Gly (G)  15	  3.1%
His (H)   9	  1.9%
Ile (I)  43	  9.0%
Leu (L)  44	  9.2%
Lys (K)  35	  7.3%
Met (M)   7	  1.5%
Phe (F)  35	  7.3%
Pro (P)  11	  2.3%
Ser (S)  46	  9.6%
Thr (T)  20	  4.2%
Trp (W)   5	  1.0%
Tyr (Y)  11	  2.3%
Val (V)  22	  4.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 72
Total number of positively charged residues (Arg + Lys): 48

Atomic composition:

Carbon      C	      2463
Hydrogen    H	      3808
Nitrogen    N	       640
Oxygen      O	       765
Sulfur      S	        16

Formula: C2463H3808N640O765S16
Total number of atoms: 7692

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    44390
Abs 0.1% (=1 g/l)   0.805, assuming all pairs of Cys residues form cystines


Ext. coefficient    43890
Abs 0.1% (=1 g/l)   0.796, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 46.30
This classifies the protein as unstable.



Aliphatic index: 87.44

Grand average of hydropathicity (GRAVY): -0.394