ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFRSITQRVI RNNCYKQSTK SITSSTSFIN NSLSYTTTSN ENDIKDKNEE HDHRAKGKGR 

        70         80         90        100        110        120 
ELLLSFDKSG LAQFPKQVFK NRKYPITDFE KYLQDITKVR GPMSIDTFIK EVLTNPKYGY 

       130        140        150        160        170        180 
YMNKDVFGKG GDFITAPEVS QLFGEMIGIW CVATWEAMGK PKKLQIVEMG PGRGTLMKDI 

       190        200        210        220        230        240 
LRSTKVFKEF YDSISVHLVE ASPANKKTQK QNLLYFKDKA INFDHKTIGE TPNGIKVTWV 

       250        260        270        280        290        300 
GKLEEVPTDI PTLFLAQEFF DALPIHVFRF SREKNDWCEV LVDEDITEHG EYYLRFVQSK 

       310        320        330        340        350        360 
GPTLMTTAVK HLLPEFGLDG YQVELGLAGL AISQQIANRI DKSGGAALII DYGYDKIVKS 

       370        380        390        400        410        420 
SLQAIRDHEF VDILDKPGTA DLSVWVDFQT IRKTVKLLKN KSTAIGPVDQ GIFLKEMGIE 

       430        440        450        460        470        480 
HRLAQIGRKL DSNEKFEELV MGYKKLVDPK EMGTNYKVIT ICDKNITPIG FSTSKTYDDE 


DLMI 

References and documentation are available.

Number of amino acids: 484

Molecular weight: 55025.20

Theoretical pI: 8.06

Amino acid composition: 
Ala (A)  20	  4.1%
Arg (R)  17	  3.5%
Asn (N)  20	  4.1%
Asp (D)  34	  7.0%
Cys (C)   4	  0.8%
Gln (Q)  18	  3.7%
Glu (E)  31	  6.4%
Gly (G)  34	  7.0%
His (H)   9	  1.9%
Ile (I)  39	  8.1%
Leu (L)  39	  8.1%
Lys (K)  50	 10.3%
Met (M)  12	  2.5%
Phe (F)  26	  5.4%
Pro (P)  18	  3.7%
Ser (S)  28	  5.8%
Thr (T)  34	  7.0%
Trp (W)   5	  1.0%
Tyr (Y)  17	  3.5%
Val (V)  29	  6.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 65
Total number of positively charged residues (Arg + Lys): 67

Atomic composition:

Carbon      C	      2482
Hydrogen    H	      3910
Nitrogen    N	       646
Oxygen      O	       732
Sulfur      S	        16

Formula: C2482H3910N646O732S16
Total number of atoms: 7786

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53080
Abs 0.1% (=1 g/l)   0.965, assuming all pairs of Cys residues form cystines


Ext. coefficient    52830
Abs 0.1% (=1 g/l)   0.960, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 23.66
This classifies the protein as stable.



Aliphatic index: 84.36

Grand average of hydropathicity (GRAVY): -0.390