ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFRSVATRLS ACRGLASNAA RKSLTIGLIP GDGIGKEVIP AGKQVLENLN SKHGLSFNFI 

        70         80         90        100        110        120 
DLYAGFQTFQ ETGKALPDET VKVLKEQCQG ALFGAVQSPT TKVEGYSSPI VALRREMGLF 

       130        140        150        160        170        180 
ANVRPVKSVE GEKGKPIDMV IVRENTEDLY IKIEKTYIDK ATGTRVADAT KRISEIATRR 

       190        200        210        220        230        240 
IATIALDIAL KRLQTRGQAT LTVTHKSNVL SQSDGLFREI CKEVYESNKD KYGQIKYNEQ 

       250        260        270        280        290        300 
IVDSMVYRLF REPQCFDVIV APNLYGDILS DGAAALVGSL GVVPSANVGP EIVIGEPCHG 

       310        320        330        340        350        360 
SAPDIAGKGI ANPIATIRST ALMLEFLGHN EAAQDIYKAV DANLREGSIK TPDLGGKAST 

       370 
QQVVDDVLSR L 

References and documentation are available.

Number of amino acids: 371

Molecular weight: 40068.94

Theoretical pI: 8.15

Amino acid composition: 
Ala (A)  35	  9.4%
Arg (R)  20	  5.4%
Asn (N)  14	  3.8%
Asp (D)  20	  5.4%
Cys (C)   5	  1.3%
Gln (Q)  15	  4.0%
Glu (E)  23	  6.2%
Gly (G)  32	  8.6%
His (H)   4	  1.1%
Ile (I)  29	  7.8%
Leu (L)  32	  8.6%
Lys (K)  25	  6.7%
Met (M)   5	  1.3%
Phe (F)  11	  3.0%
Pro (P)  15	  4.0%
Ser (S)  24	  6.5%
Thr (T)  22	  5.9%
Trp (W)   0	  0.0%
Tyr (Y)  10	  2.7%
Val (V)  30	  8.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      1769
Hydrogen    H	      2885
Nitrogen    N	       493
Oxygen      O	       543
Sulfur      S	        10

Formula: C1769H2885N493O543S10
Total number of atoms: 5700

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    15150
Abs 0.1% (=1 g/l)   0.378, assuming all pairs of Cys residues form cystines


Ext. coefficient    14900
Abs 0.1% (=1 g/l)   0.372, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.62
This classifies the protein as stable.



Aliphatic index: 97.01

Grand average of hydropathicity (GRAVY): -0.115