ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFRTKRSALV RRLWRSRAPG GEDEEEGVGG GGGGGGLRGE GATDGRAYGA GGGGAGRAGC 

        70         80         90        100        110        120 
CLGKAVRGAK GHHHPHPPSS GAGAAGGAEA DLKALTHSVL KKLKERQLEL LLQAVESRGG 

       130        140        150        160        170        180 
TRTACLLLPG RLDCRLGPGA PASAQPAQPP SSYSLPLLLC KVFRWPDLRH SSEVKRLCCC 

       190        200        210        220        230        240 
ESYGKINPEL VCCNPHHLSR LCELESPPPP YSRYPMDFLK PTADCPDAVP SSDETGGTNY 

       250        260        270        280        290        300 
LAPGGLSDSQ LLLEPGDRSH WCVVAYWEEK TRVGRLYCVQ EPSLDIFYDL PQGNGFCLGQ 

       310        320        330        340        350        360 
LNSDNKSQLV QKVRSKIGCG IQLTREVDGV WVYNRSSYPI FIKSATLDNP DSRTLLVHKV 

       370        380        390        400        410        420 
FPGFSIKAFD YEKAYSLQRP NDHEFMQQPW TGFTVQISFV KGWGQCYTRQ FISSCPCWLE 


VIFNSR 

References and documentation are available.

Number of amino acids: 426

Molecular weight: 46457.80

Theoretical pI: 8.63

Amino acid composition: 
Ala (A)  28	  6.6%
Arg (R)  30	  7.0%
Asn (N)  10	  2.3%
Asp (D)  19	  4.5%
Cys (C)  19	  4.5%
Gln (Q)  17	  4.0%
Glu (E)  23	  5.4%
Gly (G)  50	 11.7%
His (H)  11	  2.6%
Ile (I)  10	  2.3%
Leu (L)  45	 10.6%
Lys (K)  20	  4.7%
Met (M)   3	  0.7%
Phe (F)  14	  3.3%
Pro (P)  32	  7.5%
Ser (S)  35	  8.2%
Thr (T)  15	  3.5%
Trp (W)   8	  1.9%
Tyr (Y)  14	  3.3%
Val (V)  23	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 42
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      2048
Hydrogen    H	      3191
Nitrogen    N	       593
Oxygen      O	       602
Sulfur      S	        22

Formula: C2048H3191N593O602S22
Total number of atoms: 6456

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    65985
Abs 0.1% (=1 g/l)   1.420, assuming all pairs of Cys residues form cystines


Ext. coefficient    64860
Abs 0.1% (=1 g/l)   1.396, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.57
This classifies the protein as unstable.



Aliphatic index: 72.58

Grand average of hydropathicity (GRAVY): -0.398