ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFRVKIISQK RTKSVQMLEN DQLDILGQQP SLYKLYTQIC SIYRVPDPSA HDHIVNTLTR 

        70         80         90        100        110        120 
GLETLAKNFQ WLAGNVVNEG ADEGNTGTYR IVPSDKIPLI VQDLREDLSA PTMDSLEKAD 

       130        140        150        160        170        180 
FPIYMLDEKT FAPCMTINPP GNTIGMAAKS GPVFAVQANF ISGGLVLTIV GQHNIMDITG 

       190        200        210        220        230        240 
QESIINLLNK SCHQKPFSDE ELLIGNIDKS KSIPLFDETW EPDTTLVHEI VETSRNTSGE 

       250        260        270        280        290        300 
EKEQSCSSNS TWAYVEFSAI SLQNLRILAM QTCTSGTKFV STDDIVTAFI WKSVSRARLS 

       310        320        330        340        350        360 
RLKPETKSNL GRAVDVRKRL GLPETYPGLL VNMTFNTGSL KSLDHKSLGV LASQIRRKLD 

       370        380        390        400        410        420 
PKVFDLAYNT CALATLLSRC PDKTKVSIPQ PIDTLSGIMV SSWAKVSLYD VDFNLGLGKP 

       430        440        450        460        470 
KSVRRPRFIS LESLIYFMPR SSRGEMVVAL CLRDKDWECL NADKEWTNYA THIG 

References and documentation are available.

Number of amino acids: 474

Molecular weight: 52836.65

Theoretical pI: 7.03

Amino acid composition: 
Ala (A)  25	  5.3%
Arg (R)  23	  4.9%
Asn (N)  23	  4.9%
Asp (D)  29	  6.1%
Cys (C)   9	  1.9%
Gln (Q)  17	  3.6%
Glu (E)  24	  5.1%
Gly (G)  26	  5.5%
His (H)   7	  1.5%
Ile (I)  33	  7.0%
Leu (L)  50	 10.5%
Lys (K)  30	  6.3%
Met (M)  12	  2.5%
Phe (F)  16	  3.4%
Pro (P)  24	  5.1%
Ser (S)  44	  9.3%
Thr (T)  34	  7.2%
Trp (W)   7	  1.5%
Tyr (Y)  11	  2.3%
Val (V)  30	  6.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 53

Atomic composition:

Carbon      C	      2343
Hydrogen    H	      3752
Nitrogen    N	       634
Oxygen      O	       710
Sulfur      S	        21

Formula: C2343H3752N634O710S21
Total number of atoms: 7460

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    55390
Abs 0.1% (=1 g/l)   1.048, assuming all pairs of Cys residues form cystines


Ext. coefficient    54890
Abs 0.1% (=1 g/l)   1.039, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.00
This classifies the protein as stable.



Aliphatic index: 91.92

Grand average of hydropathicity (GRAVY): -0.205