ProtParam
User-provided sequence:
10 20 30 40 50 60
MFSFGRARSQ GRQNRSMSLG GLDYADPKKK NNYLGKILLT ASLTALCIFM LKQSPTFNTP
70 80 90 100 110 120
SVFSRHEPGV THVLVTGGAG YIGSHAALRL LKESYRVTIV DNLSRGNLAA VRILQELFPE
130 140 150 160 170 180
PGRLQFIYAD LGDAKAVNKI FTENAFDAVM HFAAVAYVGE STQFPLKYYH NITSNTLVVL
190 200 210 220 230 240
ETMAAHGVKT LIYSSTCATY GEPDIMPITE ETPQVPINPY GKAKKMAEDI ILDFSKNSDM
250 260 270 280 290 300
AVMILRYFNV IGSDPEGRLG EAPRPELREH GRISGACFDA ARGIMPGLQI KGTDYKTADG
310 320 330 340 350 360
TCVRDYIDVT DLVDAHVKAL QKAKPRKVGI YNVGTGKGSS VKEFVEACKK ATGVEIKIDY
370 380 390 400 410
LPRRAGDYAE VYSDPSKIRK ELNWTAKHTN LKESLETAWR WQKLHRNGYG LTTSSVSVY
References and
documentation are available.
Number of amino acids: 419
Molecular weight: 46311.07
Theoretical pI: 9.14
Total number of negatively charged residues (Asp + Glu): 43
Total number of positively charged residues (Arg + Lys): 53
Atomic composition:
Carbon C 2069
Hydrogen H 3270
Nitrogen N 568
Oxygen O 608
Sulfur S 15
Formula: C
2069H
3270N
568O
608S
15
Total number of atoms: 6530
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 46550
Abs 0.1% (=1 g/l) 1.005, assuming all pairs of Cys residues form cystines
Ext. coefficient 46300
Abs 0.1% (=1 g/l) 1.000, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.18
This classifies the protein as stable.
Aliphatic index: 83.82
Grand average of hydropathicity (GRAVY): -0.259