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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFSFGRARSQ GRQNRSMSLG GLDYADPKKK NNYLGKILLT ASLTALCIFM LKQSPTFNTP

70 80 90 100 110 120
SVFSRHEPGV THVLVTGGAG YIGSHAALRL LKESYRVTIV DNLSRGNLAA VRILQELFPE

130 140 150 160 170 180
PGRLQFIYAD LGDAKAVNKI FTENAFDAVM HFAAVAYVGE STQFPLKYYH NITSNTLVVL

190 200 210 220 230 240
ETMAAHGVKT LIYSSTCATY GEPDIMPITE ETPQVPINPY GKAKKMAEDI ILDFSKNSDM

250 260 270 280 290 300
AVMILRYFNV IGSDPEGRLG EAPRPELREH GRISGACFDA ARGIMPGLQI KGTDYKTADG

310 320 330 340 350 360
TCVRDYIDVT DLVDAHVKAL QKAKPRKVGI YNVGTGKGSS VKEFVEACKK ATGVEIKIDY

370 380 390 400 410
LPRRAGDYAE VYSDPSKIRK ELNWTAKHTN LKESLETAWR WQKLHRNGYG LTTSSVSVY


References and documentation are available.

Number of amino acids: 419 Molecular weight: 46311.07 Theoretical pI: 9.14
Amino acid composition: 
Ala (A) 37 8.8% Arg (R) 23 5.5% Asn (N) 17 4.1% Asp (D) 21 5.0% Cys (C) 5 1.2% Gln (Q) 10 2.4% Glu (E) 22 5.3% Gly (G) 34 8.1% His (H) 10 2.4% Ile (I) 24 5.7% Leu (L) 35 8.4% Lys (K) 30 7.2% Met (M) 10 2.4% Phe (F) 15 3.6% Pro (P) 19 4.5% Ser (S) 27 6.4% Thr (T) 28 6.7% Trp (W) 3 0.7% Tyr (Y) 20 4.8% Val (V) 29 6.9% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 43 Total number of positively charged residues (Arg + Lys): 53 Atomic composition: Carbon C 2069 Hydrogen H 3270 Nitrogen N 568 Oxygen O 608 Sulfur S 15 Formula: C2069H3270N568O608S15 Total number of atoms: 6530 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 46550 Abs 0.1% (=1 g/l) 1.005, assuming all pairs of Cys residues form cystines Ext. coefficient 46300 Abs 0.1% (=1 g/l) 1.000, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 36.18 This classifies the protein as stable. Aliphatic index: 83.82 Grand average of hydropathicity (GRAVY): -0.259