ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFSGFSSFGG GNGFVNMPQT FEEFFRCYPI AMMNDRIRKD DANFGGKIFL PPSALSKLSM 

        70         80         90        100        110        120 
LNIRYPMLFK LTANETGRVT HGGVLEFIAE EGRVYLPQWM METLGIQPGS LLQISSTDVP 

       130        140        150        160        170        180 
LGQFVKLEPQ SVDFLDISDP KAVLENVLRN FSTLTVDDVI EISYNGKTFK IKILEVKPES 

       190        200        210        220        230        240 
SSKSICVIET DLVTDFAPPV GYVEPDYKAL KAQQDKEKKN SFGKGQVLDP SVLGQGSMST 

       250        260        270        280        290        300 
RIDYAGIANS SRNKLSKFVG QGQNISGKAP KAEPKQDIKD MKITFDGEPA KLDLPEGQLF 

       310        320        330        340        350        360 
FGFPMVLPKE DEESAAGSKS SEQNFQGQGI SLRKSNKRKT KSDHDSSKSK APKSPEVIEI 


D 

References and documentation are available.

Number of amino acids: 361

Molecular weight: 39810.39

Theoretical pI: 5.94

Amino acid composition: 
Ala (A)  17	  4.7%
Arg (R)  11	  3.0%
Asn (N)  15	  4.2%
Asp (D)  23	  6.4%
Cys (C)   2	  0.6%
Gln (Q)  17	  4.7%
Glu (E)  24	  6.6%
Gly (G)  30	  8.3%
His (H)   2	  0.6%
Ile (I)  22	  6.1%
Leu (L)  28	  7.8%
Lys (K)  34	  9.4%
Met (M)  11	  3.0%
Phe (F)  23	  6.4%
Pro (P)  23	  6.4%
Ser (S)  36	 10.0%
Thr (T)  14	  3.9%
Trp (W)   1	  0.3%
Tyr (Y)   7	  1.9%
Val (V)  21	  5.8%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 45

Atomic composition:

Carbon      C	      1776
Hydrogen    H	      2807
Nitrogen    N	       465
Oxygen      O	       545
Sulfur      S	        13

Formula: C1776H2807N465O545S13
Total number of atoms: 5606

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    16055
Abs 0.1% (=1 g/l)   0.403, assuming all pairs of Cys residues form cystines


Ext. coefficient    15930
Abs 0.1% (=1 g/l)   0.400, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.62
This classifies the protein as unstable.



Aliphatic index: 75.60

Grand average of hydropathicity (GRAVY): -0.410