ProtParam
User-provided sequence:
10 20 30 40 50 60
MFSHYRYKEN SCQKREAIPD KSRVSLTFLQ KRTDSSNVTV AVAVAVPIGA IIIVLSVVLI
70 80 90 100 110 120
VVYRRCKKNL LCRILTQILR AIYTIYRRWI TSMNSANSDS NATEKRFIYG GYDDFLQPSI
130 140 150 160 170 180
ENSQSFKDYV RRINEHAPSA YNIASLASQN NSKLSVPSKH IDLSNKISFE SLENSELIVS
190 200 210 220 230 240
PQHSNTGQDC DQRCDSTSNP DVNEKSSHNN DNRLKSNYTS RSGLEPQCSR EEEENIDRIR
250 260 270 280 290 300
SIYNIYFEKS NSTIRSSVTS SIRRDSKLNI ATRKSVNMSS QDNPNDTTLI EQSHFGSTTV
310 320 330 340 350 360
QEIDSSSSAN EEYEDATDYL QVPAPQENKN IASSVYSEVA TREKVIPESS LSLTFPPPNG
370 380 390 400 410 420
LSTRITSSIY SDTVAKDHIH SAKAPVRALS EGSGQSNLTS AQQYSTYFID HCNQSNDDNY
430 440 450 460 470 480
YYNYPLPLEH PQNYENIGDL PTPTQFIYST SSHSLTSFKG RPKPPKTLKH VPTARLNGTA
490 500 510 520 530 540
LNPMDHPEMF YSSPTKIPST SLTKQFCTPL PYQLRQSVVM TNPSELSMKP RYKPAGSLRN
550 560 570 580 590 600
LIKAQYLPGN SSTTTSSSLS QPPSTLSNAI NFRVSGLLDD TDILQPPSVG EILPFKASTE
610 620
DLRKQLGTSH NYEITPYENV HV
References and
documentation are available.
Number of amino acids: 622
Molecular weight: 69625.43
Theoretical pI: 7.61
Total number of negatively charged residues (Asp + Glu): 63
Total number of positively charged residues (Arg + Lys): 64
Atomic composition:
Carbon C 3033
Hydrogen H 4788
Nitrogen N 858
Oxygen O 992
Sulfur S 15
Formula: C
3033H
4788N
858O
992S
15
Total number of atoms: 9686
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 50700
Abs 0.1% (=1 g/l) 0.728, assuming all pairs of Cys residues form cystines
Ext. coefficient 50200
Abs 0.1% (=1 g/l) 0.721, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 60.96
This classifies the protein as unstable.
Aliphatic index: 73.67
Grand average of hydropathicity (GRAVY): -0.641