ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFSIFNSPCV FEQLPSFSQP LHSRYFDCSS PVSYYPECKR RKAIKANLRA PKKSDANCSE 

        70         80         90        100        110        120 
PLRYALAETP NGYTLSLSKR IPYELFSKYV NEKLGELKEN HYRPTYHVVQ DFFGNQYYVE 

       130        140        150        160        170        180 
DEADEDALLR SALKDLDFRA IGKKIAKDLF QDYEIELNHR GDELSILSKK DKIFKEFSLD 

       190        200        210        220        230        240 
QVFEDVFVIG CGVENIDDGS REKYALLKIG LVKHEEEISE GGINEPKMPI IESKIDESHD 

       250        260        270        280        290        300 
DVNMSESLKE EEAEKAKEPL TKEDQIKKWI EEERLMQEES RKSEQEKAAK EDEERQKKEK 

       310        320        330        340        350        360 
EARLKARKES LINKQKTKRS QQKKLQNSKS LPISEIEASN KNNNSNSGSA ESDNESINSD 

       370        380        390        400        410 
SDTTLDFSVS GNTLKKHASP LLEDVEDEEV DRYNESLSRS PKGNSIIEEI 

References and documentation are available.

Number of amino acids: 410

Molecular weight: 47167.53

Theoretical pI: 5.09

Amino acid composition: 
Ala (A)  21	  5.1%
Arg (R)  19	  4.6%
Asn (N)  23	  5.6%
Asp (D)  28	  6.8%
Cys (C)   5	  1.2%
Gln (Q)  14	  3.4%
Glu (E)  56	 13.7%
Gly (G)  14	  3.4%
His (H)   7	  1.7%
Ile (I)  25	  6.1%
Leu (L)  34	  8.3%
Lys (K)  46	 11.2%
Met (M)   4	  1.0%
Phe (F)  15	  3.7%
Pro (P)  16	  3.9%
Ser (S)  45	 11.0%
Thr (T)   8	  2.0%
Trp (W)   1	  0.2%
Tyr (Y)  14	  3.4%
Val (V)  15	  3.7%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 84
Total number of positively charged residues (Arg + Lys): 65

Atomic composition:

Carbon      C	      2060
Hydrogen    H	      3269
Nitrogen    N	       565
Oxygen      O	       683
Sulfur      S	         9

Formula: C2060H3269N565O683S9
Total number of atoms: 6586

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26610
Abs 0.1% (=1 g/l)   0.564, assuming all pairs of Cys residues form cystines


Ext. coefficient    26360
Abs 0.1% (=1 g/l)   0.559, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 61.59
This classifies the protein as unstable.



Aliphatic index: 71.85

Grand average of hydropathicity (GRAVY): -0.971