ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFSKLPIQAV RGAASAIRTH QIPGHRLPLA KYGGRHTVCA LPGDGIGPEM IAHIRNIFSF 

        70         80         90        100        110        120 
CHAPVNFEEV QVSSSLLDGD MDAAMLAIER NGVAIKGNIE TKHDDPQFNS RNVELRTKLD 

       130        140        150        160        170        180 
LYANILHCVT IPTVPTRHSG IDIVLIRENT EGEYSGLEHE AVPGIVESIK IVTREKIERI 

       190        200        210        220        230        240 
SRMAFEYAKA NGRKKVTAVH KANIQKLGDG LFLKVVRDMS EDYKDIKFEA MIVDNASMQL 

       250        260        270        280        290        300 
VSKPQQFDVM VMPNLYGNII SNIACGLVGG PGLVSGMNLG DKYAVFETGT RNTGTSLAGK 

       310        320        330        340        350        360 
DIANPTAFIR ASVDMLRYLG CHYHANIISD ALWKALVEQR IHTADIGGNN SASDVINATL 

       370 
QNIKVLMDEN PKH 

References and documentation are available.

Number of amino acids: 373

Molecular weight: 40849.94

Theoretical pI: 6.92

Amino acid composition: 
Ala (A)  33	  8.8%
Arg (R)  19	  5.1%
Asn (N)  23	  6.2%
Asp (D)  21	  5.6%
Cys (C)   5	  1.3%
Gln (Q)  10	  2.7%
Glu (E)  20	  5.4%
Gly (G)  30	  8.0%
His (H)  14	  3.8%
Ile (I)  34	  9.1%
Leu (L)  28	  7.5%
Lys (K)  21	  5.6%
Met (M)  13	  3.5%
Phe (F)  11	  2.9%
Pro (P)  15	  4.0%
Ser (S)  21	  5.6%
Thr (T)  17	  4.6%
Trp (W)   1	  0.3%
Tyr (Y)   9	  2.4%
Val (V)  28	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 41
Total number of positively charged residues (Arg + Lys): 40

Atomic composition:

Carbon      C	      1798
Hydrogen    H	      2893
Nitrogen    N	       513
Oxygen      O	       536
Sulfur      S	        18

Formula: C1798H2893N513O536S18
Total number of atoms: 5758

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    19160
Abs 0.1% (=1 g/l)   0.469, assuming all pairs of Cys residues form cystines


Ext. coefficient    18910
Abs 0.1% (=1 g/l)   0.463, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.88
This classifies the protein as stable.



Aliphatic index: 95.44

Grand average of hydropathicity (GRAVY): -0.118