ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFSLKALLPL ALLLVSANQV AAKVHKAKIY KHELSDEMKE VTFEQHLAHL GQKYLTQFEK 

        70         80         90        100        110        120 
ANPEVVFSRE HPFFTEGGHD VPLTNYLNAQ YYTDITLGTP PQNFKVILDT GSSNLWVPSN 

       130        140        150        160        170        180 
ECGSLACFLH SKYDHEASSS YKANGTEFAI QYGTGSLEGY ISQDTLSIGD LTIPKQDFAE 

       190        200        210        220        230        240 
ATSEPGLTFA FGKFDGILGL GYDTISVDKV VPPFYNAIQQ DLLDEKRFAF YLGDTSKDTE 

       250        260        270        280        290        300 
NGGEATFGGI DESKFKGDIT WLPVRRKAYW EVKFEGIGLG DEYAELESHG AAIDTGTSLI 

       310        320        330        340        350        360 
TLPSGLAEMI NAEIGAKKGW TGQYTLDCNT RDNLPDLIFN FNGYNFTIGP YDYTLEVSGS 

       370        380        390        400 
CISAITPMDF PEPVGPLAIV GDAFLRKYYS IYDLGNNAVG LAKAI 

References and documentation are available.

Number of amino acids: 405

Molecular weight: 44499.00

Theoretical pI: 4.70

Amino acid composition: 
Ala (A)  32	  7.9%
Arg (R)   6	  1.5%
Asn (N)  18	  4.4%
Asp (D)  26	  6.4%
Cys (C)   4	  1.0%
Gln (Q)  12	  3.0%
Glu (E)  27	  6.7%
Gly (G)  38	  9.4%
His (H)   9	  2.2%
Ile (I)  24	  5.9%
Leu (L)  41	 10.1%
Lys (K)  24	  5.9%
Met (M)   4	  1.0%
Phe (F)  24	  5.9%
Pro (P)  19	  4.7%
Ser (S)  26	  6.4%
Thr (T)  28	  6.9%
Trp (W)   4	  1.0%
Tyr (Y)  21	  5.2%
Val (V)  18	  4.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 53
Total number of positively charged residues (Arg + Lys): 30

Atomic composition:

Carbon      C	      2023
Hydrogen    H	      3059
Nitrogen    N	       499
Oxygen      O	       617
Sulfur      S	         8

Formula: C2023H3059N499O617S8
Total number of atoms: 6206

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    53540
Abs 0.1% (=1 g/l)   1.203, assuming all pairs of Cys residues form cystines


Ext. coefficient    53290
Abs 0.1% (=1 g/l)   1.198, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 26.35
This classifies the protein as stable.



Aliphatic index: 83.38

Grand average of hydropathicity (GRAVY): -0.185