ProtParam
User-provided sequence:
10 20 30 40 50 60
MFSRLPTSLA RNVARRAPTS FVRPSAAAAA LRFSSTKTMT VREALNSAMA EELDRDDDVF
70 80 90 100 110 120
LIGEEVAQYN GAYKVSKGLL DRFGERRVVD TPITEYGFTG LAVGAALKGL KPIVEFMSFN
130 140 150 160 170 180
FSMQAIDHVV NSAAKTHYMS GGTQKCQMVF RGPNGAAVGV GAQHSQDFSP WYGSIPGLKV
190 200 210 220 230 240
LVPYSAEDAR GLLKAAIRDP NPVVFLENEL LYGESFEISE EALSPEFTLP YKAKIEREGT
250 260 270 280 290 300
DISIVTYTRN VQFSLEAAEI LQKKYGVSAE VINLRSIRPL DTEAIIKTVK KTNHLITVES
310 320 330 340 350 360
TFPSFGVGAE IVAQVMESEA FDYLDAPIQR VTGADVPTPY AKELEDFAFP DTPTIVKAVK
EVLSIE
References and
documentation are available.
Number of amino acids: 366
Molecular weight: 40053.64
Theoretical pI: 5.21
Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 38
Atomic composition:
Carbon C 1798
Hydrogen H 2832
Nitrogen N 472
Oxygen O 544
Sulfur S 9
Formula: C
1798H
2832N
472O
544S
9
Total number of atoms: 5655
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 23380
Abs 0.1% (=1 g/l) 0.584, assuming all pairs of Cys residues form cystines
Ext. coefficient 23380
Abs 0.1% (=1 g/l) 0.584, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.71
This classifies the protein as stable.
Aliphatic index: 88.22
Grand average of hydropathicity (GRAVY): -0.069