ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFSRSTLCLK TSASSIGRLQ LRYFSHLPMT VPIKLPNGLE YEQPTGLFIN NKFVPSKQNK 

        70         80         90        100        110        120 
TFEVINPSTE EEICHIYEGR EDDVEEAVQA ADRAFSNGSW NGIDPIDRGK ALYRLAELIE 

       130        140        150        160        170        180 
QDKDVIASIE TLDNGKAISS SRGDVDLVIN YLKSSAGFAD KIDGRMIDTG RTHFSYTKRQ 

       190        200        210        220        230        240 
PLGVCGQIIP WNFPLLMWAW KIAPALVTGN TVVLKTAEST PLSALYVSKY IPQAGIPPGV 

       250        260        270        280        290        300 
INIVSGFGKI VGEAITNHPK IKKVAFTGST ATGRHIYQSA AAGLKKVTLE LGGKSPNIVF 

       310        320        330        340        350        360 
ADAELKKAVQ NIILGIYYNS GEVCCAGSRV YVEESIYDKF IEEFKAASES IKVGDPFDES 

       370        380        390        400        410        420 
TFQGAQTSQM QLNKILKYVD IGKNEGATLI TGGERLGSKG YFIKPTVFGD VKEDMRIVKE 

       430        440        450        460        470        480 
EIFGPVVTVT KFKSADEVIN MANDSEYGLA AGIHTSNINT ALKVADRVNA GTVWINTYND 

       490        500        510 
FHHAVPFGGF NASGLGREMS VDALQNYLQV KAVRAKLDE 

References and documentation are available.

Number of amino acids: 519

Molecular weight: 56723.66

Theoretical pI: 6.30

Amino acid composition: 
Ala (A)  43	  8.3%
Arg (R)  18	  3.5%
Asn (N)  27	  5.2%
Asp (D)  26	  5.0%
Cys (C)   5	  1.0%
Gln (Q)  16	  3.1%
Glu (E)  33	  6.4%
Gly (G)  45	  8.7%
His (H)   8	  1.5%
Ile (I)  43	  8.3%
Leu (L)  36	  6.9%
Lys (K)  38	  7.3%
Met (M)   8	  1.5%
Phe (F)  23	  4.4%
Pro (P)  21	  4.0%
Ser (S)  37	  7.1%
Thr (T)  30	  5.8%
Trp (W)   5	  1.0%
Tyr (Y)  18	  3.5%
Val (V)  39	  7.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 59
Total number of positively charged residues (Arg + Lys): 56

Atomic composition:

Carbon      C	      2544
Hydrogen    H	      4009
Nitrogen    N	       675
Oxygen      O	       766
Sulfur      S	        13

Formula: C2544H4009N675O766S13
Total number of atoms: 8007

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    54570
Abs 0.1% (=1 g/l)   0.962, assuming all pairs of Cys residues form cystines


Ext. coefficient    54320
Abs 0.1% (=1 g/l)   0.958, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 31.42
This classifies the protein as stable.



Aliphatic index: 89.44

Grand average of hydropathicity (GRAVY): -0.151