ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFSSSSRPSK EPLLFDIRLR NLDNDVLLIK GPPDEASSVL LSGTIVLSIT EPIQIKSLAL 

        70         80         90        100        110        120 
RLFGRLRLNI PTVLQTVHGP HKRYSKFERN IYSHFWDDFN IKSYFQNLYD NHNNGKITIS 

       130        140        150        160        170        180 
SKSSTNLAAL PKRKRALSTA SLISSNGQTS ASKNYHTLVK GNYEFPFSAI IPGSLVESVE 

       190        200        210        220        230        240 
GLPNAAVTYA LEATIERPKQ PDLICKKHLR VIRTLAIDAV ELSETVSVDN SWPEKVDYTI 

       250        260        270        280        290        300 
SIPTKAIAIG SSTMINILIV PILKGLKLGP VRISLVENSQ YCGSYGGVIN QERMVAKLKL 

       310        320        330        340        350        360 
KDPLKHVAQI KKKRSLNEAA DEGVDTDTGE FQDKWEVRAL LNIPASLTKC SQDCRILSNI 

       370        380        390        400        410        420 
KVRHKIKFTI SLLNPDGHIS ELRAALPVQL FISPFVPVNV KTSDVIERTL KTFGPSYQVT 

       430        440        450        460        470        480 
SQHDNSFSSK NFVDDSEEDV IFQRSASALQ LSSMPTIVSG STLNINSTDA EATAVADTTM 

       490        500        510        520        530        540 
VTSLMVPPNY GNHVYDRVYG EVTNEDETSA SASSSAVESQ AIHNIQNLYI SDSNNSNNPI 

       550        560        570        580        590        600 
LAPNPQIKIE DDSLNNCDSR GDSVNNSNLN LVNSNLTISE NWNNNSPSAN RYNNIINAGL 

       610        620        630        640        650        660 
NSPSLTPSFA HLSRRNSYSR QTSSTSLKND LELTDLSRVP SYDKAMKSDM IGEDLPPAYP 

       670        680        690        700        710        720 
EEELGVQENK KIELERPQIL HHKSTSSLLP LPGSSKSSNN LKRSSSRTHL SHSPLPRNNS 

       730        740        750        760        770        780 
GSSVSLQQLA RNNTDSSFNL NLSFTSAKSS TGSRHFPFNM TTSFTSNSSS KNNSHFDKTD 

       790        800        810        820        830 
STSDANKPRE EENYTSATHN RRSRSSSVRS NNSNSPLRQG TGSFANLMEM FTKRDRS 

References and documentation are available.

Number of amino acids: 837

Molecular weight: 92349.33

Theoretical pI: 8.88

Amino acid composition: 
Ala (A)  45	  5.4%
Arg (R)  43	  5.1%
Asn (N)  76	  9.1%
Asp (D)  42	  5.0%
Cys (C)   5	  0.6%
Gln (Q)  24	  2.9%
Glu (E)  41	  4.9%
Gly (G)  31	  3.7%
His (H)  20	  2.4%
Ile (I)  52	  6.2%
Leu (L)  80	  9.6%
Lys (K)  48	  5.7%
Met (M)  11	  1.3%
Phe (F)  26	  3.1%
Pro (P)  45	  5.4%
Ser (S) 127	 15.2%
Thr (T)  52	  6.2%
Trp (W)   4	  0.5%
Tyr (Y)  20	  2.4%
Val (V)  45	  5.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 83
Total number of positively charged residues (Arg + Lys): 91

Atomic composition:

Carbon      C	      4019
Hydrogen    H	      6446
Nitrogen    N	      1158
Oxygen      O	      1303
Sulfur      S	        16

Formula: C4019H6446N1158O1303S16
Total number of atoms: 12942

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    52050
Abs 0.1% (=1 g/l)   0.564, assuming all pairs of Cys residues form cystines


Ext. coefficient    51800
Abs 0.1% (=1 g/l)   0.561, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.83
This classifies the protein as unstable.



Aliphatic index: 82.47

Grand average of hydropathicity (GRAVY): -0.505