ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFSVTRRRAA GAAAAMATAT GTLYWMTSQG DRPLVHNDPS YMVQFPTAAP PQVSRRDLLD 

        70         80         90        100        110        120 
RLAKTHQFDV LIIGGGATGT GCALDAATRG LNVALVEKGD FASGTSSKST KMIHGGVRYL 

       130        140        150        160        170        180 
EKAFWEFSKA QLDLVIEALN ERKHLINTAP HLCTVLPILI PIYSTWQVPY IYMGCKFYDF 

       190        200        210        220        230        240 
FAGSQNLKKS YLLSKSATVE KAPMLTTDNL KASLVYHDGS FNDSRLNATL AITAVENGAT 

       250        260        270        280        290        300 
VLNYVEVQKL IKDPTSGKVI GAEARDVETN ELVRINAKCV VNATGPYSDA ILQMDRNPSG 

       310        320        330        340        350        360 
LPDSPLNDNS KIKSTFNQIA VMDPKMVIPS IGVHIVLPSF YCPKDMGLLD VRTSDGRVMF 

       370        380        390        400        410        420 
FLPWQGKVLA GTTDIPLKQV PENPMPTEAD IQDILKELQH YIEFPVKRED VLSAWAGVRP 

       430        440        450        460        470        480 
LVRDPRTIPA DGKKGSATQG VVRSHFLFTS DNGLITIAGG KWTTYRQMAE ETVDKVVEVG 

       490        500        510        520        530        540 
GFHNLKPCHT RDIKLAGAEE WTQNYVALLA QNYHLSSKMS NYLVQNYGTR SSIICEFFKE 

       550        560        570        580        590        600 
SMENKLPLSL ADKENNVIYS SEENNLVNFD TFRYPFTIGE LKYSMQYEYC RTPLDFLLRR 

       610        620        630        640 
TRFAFLDAKE ALNAVHATVK VMGDEFNWSE KKRQWELEKT VNFIKTFGV 

References and documentation are available.

Number of amino acids: 649

Molecular weight: 72388.93

Theoretical pI: 7.96

Amino acid composition: 
Ala (A)  53	  8.2%
Arg (R)  30	  4.6%
Asn (N)  33	  5.1%
Asp (D)  36	  5.5%
Cys (C)   8	  1.2%
Gln (Q)  21	  3.2%
Glu (E)  34	  5.2%
Gly (G)  40	  6.2%
His (H)  13	  2.0%
Ile (I)  32	  4.9%
Leu (L)  60	  9.2%
Lys (K)  42	  6.5%
Met (M)  18	  2.8%
Phe (F)  29	  4.5%
Pro (P)  32	  4.9%
Ser (S)  41	  6.3%
Thr (T)  46	  7.1%
Trp (W)   9	  1.4%
Tyr (Y)  23	  3.5%
Val (V)  49	  7.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 70
Total number of positively charged residues (Arg + Lys): 72

Atomic composition:

Carbon      C	      3245
Hydrogen    H	      5090
Nitrogen    N	       870
Oxygen      O	       954
Sulfur      S	        26

Formula: C3245H5090N870O954S26
Total number of atoms: 10185

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    84270
Abs 0.1% (=1 g/l)   1.164, assuming all pairs of Cys residues form cystines


Ext. coefficient    83770
Abs 0.1% (=1 g/l)   1.157, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.20
This classifies the protein as stable.



Aliphatic index: 85.35

Grand average of hydropathicity (GRAVY): -0.210