ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFTGQEYHSV DSNSNKQKDN NKRGIDDTSK ILNNKIPHSV SDTSAAATTT STMNNSALSR 

        70         80         90        100        110        120 
SLDPTDINYS TNMAGVVDQI HDYTTSNRNS LTPQYSIAAG NVNSHDRVVK PSANSNYQQA 

       130        140        150        160        170        180 
AYLRQQQQQD QRQQSPSMKT EEESQLYGDI LMNSGVVQDM HQNLATHTNL SQLSSTRKSA 

       190        200        210        220        230        240 
PNDSTTAPTN ASNIANTASV NKQMYFMNMN MNNNPHALND PSILETLSPF FQPFGVDVAH 

       250        260        270        280        290        300 
LPMTNPPIFQ SSLPGCDEPI RRRRISISNG QISQLGEDIE TLENLHNTQP PPMPNFHNYN 

       310        320        330        340        350        360 
GLSQTRNVSN KPVFNQAVPV SSIPQYNAKK VINPTKDSAL GDQSVIYSKS QQRNFVNAPS 

       370        380        390        400        410        420 
KNTPAESISD LEGMTTFAPT TGGENRGKSA LRESHSNPSF TPKSQGSHLN LAANTQGNPI 

       430        440        450        460        470        480 
PGTTAWKRAR LLERNRIAAS KCRQRKKVAQ LQLQKEFNEI KDENRILLKK LNYYEKLISK 

       490        500        510        520        530        540 
FKKFSKIHLR EHEKLNKDSD NNVNGTNSSN KNESMTVDSL KIIEELLMID SDVTEVDKDT 

       550        560        570        580 
GKIIAIKHEP YSQRFGSDTD DDDIDLKPVE GGKDPDNQSL PNSEKIK 

References and documentation are available.

Number of amino acids: 587

Molecular weight: 65264.25

Theoretical pI: 7.75

Amino acid composition: 
Ala (A)  33	  5.6%
Arg (R)  24	  4.1%
Asn (N)  63	 10.7%
Asp (D)  37	  6.3%
Cys (C)   2	  0.3%
Gln (Q)  38	  6.5%
Glu (E)  27	  4.6%
Gly (G)  24	  4.1%
His (H)  15	  2.6%
Ile (I)  33	  5.6%
Leu (L)  39	  6.6%
Lys (K)  41	  7.0%
Met (M)  15	  2.6%
Phe (F)  15	  2.6%
Pro (P)  35	  6.0%
Ser (S)  66	 11.2%
Thr (T)  40	  6.8%
Trp (W)   1	  0.2%
Tyr (Y)  14	  2.4%
Val (V)  25	  4.3%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 64
Total number of positively charged residues (Arg + Lys): 65

Atomic composition:

Carbon      C	      2795
Hydrogen    H	      4468
Nitrogen    N	       832
Oxygen      O	       937
Sulfur      S	        17

Formula: C2795H4468N832O937S17
Total number of atoms: 9049

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    26485
Abs 0.1% (=1 g/l)   0.406, assuming all pairs of Cys residues form cystines


Ext. coefficient    26360
Abs 0.1% (=1 g/l)   0.404, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.65
This classifies the protein as unstable.



Aliphatic index: 65.81

Grand average of hydropathicity (GRAVY): -0.890