ProtParam
User-provided sequence:
10 20 30 40 50 60
MFVSPPPATA RNQVLGKRKS KRHDENPKNV QPNADTEMTN SVPSIGFNSN LPHNNQEINT
70 80 90 100 110 120
PNHYNLSSNS GNVRSNNNFV TTPPEYADRA RIEIIKRLLP TAGTKPMEVN SNTAENANIQ
130 140 150 160 170 180
HINTPDSQSF VSDHSSSYES SIFSQPSTAL TDITTGSSLI DTKTPKFVTE VTLEDALPKT
190 200 210 220 230 240
FYDMYSPEVL MSDPANILYN GRPKFTKREL LDWDLNDIRS LLIVEQLRPE WGSQLPTVVT
250 260 270 280 290 300
SGINLPQFRL QLLPLSSSDE FIIATLVNSD LYIEANLDRN FKLTSAKYTV ASARKRHEEM
310 320 330 340 350 360
TGSKEPIMRL SKPEWRNIIE NYLLNVAVEA QCRYDFKQKR SEYKRWKLLN SNLKRPDMPP
370 380 390 400 410 420
PSLIPHGFKI HDCTNSGSLL KKALMKNLQL KNYKNDAKTL GAGTQKNVVN KVSLTSEERA
430 440
AIWFQCQTQV YQRLGLDWKP DGMS
References and
documentation are available.
Number of amino acids: 444
Molecular weight: 50260.84
Theoretical pI: 9.05
Total number of negatively charged residues (Asp + Glu): 47
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 2216
Hydrogen H 3516
Nitrogen N 624
Oxygen O 684
Sulfur S 13
Formula: C
2216H
3516N
624O
684S
13
Total number of atoms: 7053
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 52495
Abs 0.1% (=1 g/l) 1.044, assuming all pairs of Cys residues form cystines
Ext. coefficient 52370
Abs 0.1% (=1 g/l) 1.042, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 52.29
This classifies the protein as unstable.
Aliphatic index: 77.52
Grand average of hydropathicity (GRAVY): -0.642