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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFVSPPPATA RNQVLGKRKS KRHDENPKNV QPNADTEMTN SVPSIGFNSN LPHNNQEINT

70 80 90 100 110 120
PNHYNLSSNS GNVRSNNNFV TTPPEYADRA RIEIIKRLLP TAGTKPMEVN SNTAENANIQ

130 140 150 160 170 180
HINTPDSQSF VSDHSSSYES SIFSQPSTAL TDITTGSSLI DTKTPKFVTE VTLEDALPKT

190 200 210 220 230 240
FYDMYSPEVL MSDPANILYN GRPKFTKREL LDWDLNDIRS LLIVEQLRPE WGSQLPTVVT

250 260 270 280 290 300
SGINLPQFRL QLLPLSSSDE FIIATLVNSD LYIEANLDRN FKLTSAKYTV ASARKRHEEM

310 320 330 340 350 360
TGSKEPIMRL SKPEWRNIIE NYLLNVAVEA QCRYDFKQKR SEYKRWKLLN SNLKRPDMPP

370 380 390 400 410 420
PSLIPHGFKI HDCTNSGSLL KKALMKNLQL KNYKNDAKTL GAGTQKNVVN KVSLTSEERA

430 440
AIWFQCQTQV YQRLGLDWKP DGMS


References and documentation are available.

Number of amino acids: 444 Molecular weight: 50260.84 Theoretical pI: 9.05
Amino acid composition: 
Ala (A) 23 5.2% Arg (R) 23 5.2% Asn (N) 40 9.0% Asp (D) 22 5.0% Cys (C) 3 0.7% Gln (Q) 18 4.1% Glu (E) 25 5.6% Gly (G) 15 3.4% His (H) 8 1.8% Ile (I) 23 5.2% Leu (L) 43 9.7% Lys (K) 31 7.0% Met (M) 10 2.3% Phe (F) 14 3.2% Pro (P) 31 7.0% Ser (S) 43 9.7% Thr (T) 31 7.0% Trp (W) 6 1.4% Tyr (Y) 13 2.9% Val (V) 22 5.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 47 Total number of positively charged residues (Arg + Lys): 54 Atomic composition: Carbon C 2216 Hydrogen H 3516 Nitrogen N 624 Oxygen O 684 Sulfur S 13 Formula: C2216H3516N624O684S13 Total number of atoms: 7053 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 52495 Abs 0.1% (=1 g/l) 1.044, assuming all pairs of Cys residues form cystines Ext. coefficient 52370 Abs 0.1% (=1 g/l) 1.042, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 52.29 This classifies the protein as unstable. Aliphatic index: 77.52 Grand average of hydropathicity (GRAVY): -0.642