ProtParam
User-provided sequence:
10 20 30 40 50 60
MFVSPPPATS KNQVLQRRPL ESTNSNHGFA SSLQAIPENT MSGSDNASFQ SLPLSMSSSQ
70 80 90 100 110 120
STTSSRRENF VNAPPEYTDR ARDEIKKRLL ASSPSRRSHH SSSMHSASRR SSVAESGSLL
130 140 150 160 170 180
SDNASSYQSS IFSAPSTVHT QLTNDSSFSE FPNHKLITRV SLDEALPKTF YDMYSPDILL
190 200 210 220 230 240
ADPSNILCNG RPKFTKRELL DWDLNDIRSL LIVEKLRPEW GNQLPEVITV GDNMPQFRLQ
250 260 270 280 290 300
LLPLYSSDET IIATLVHSDL YMEANLDYEF KLTSAKYTVA TARKRHEHIT GRNEAVMNLS
310 320 330 340 350 360
KPEWRNIIEN YLLNIAVEAQ CRFDFKQRCS EYKKWKLQQS NLKRPDMPPP SIIPRKNSTE
370 380 390 400 410 420
TKSLLKKALL KNIQLKNPNN NLDELMMRSS AATNQQGKNK VSLSKEEKAT IWSQCQAQVY
430
QRLGLDWQPD SVS
References and
documentation are available.
Number of amino acids: 433
Molecular weight: 49060.31
Theoretical pI: 9.14
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 54
Atomic composition:
Carbon C 2142
Hydrogen H 3415
Nitrogen N 617
Oxygen O 673
Sulfur S 15
Formula: C
2142H
3415N
617O
673S
15
Total number of atoms: 6862
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 49640
Abs 0.1% (=1 g/l) 1.012, assuming all pairs of Cys residues form cystines
Ext. coefficient 49390
Abs 0.1% (=1 g/l) 1.007, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 65.37
This classifies the protein as unstable.
Aliphatic index: 75.27
Grand average of hydropathicity (GRAVY): -0.666