ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFYNQDVLTK EKGGMGVIWL AATLGSKHSL RKLHKKDIMS VDIDEACDFV AFSPEPLALR 

        70         80         90        100        110        120 
LSSNLMIGVT RVWAHQYSFF HSQVSTLHLR VRKELDHFTS KPFKNIDIQN EQTNPKQLLL 

       130        140        150        160        170        180 
AEDPAFIPEV SLYDAFNLPS VDLHVDMSSF TQPKENPNIS VLETLPDSTS YLINTSQNYS 

       190        200        210        220        230        240 
LRNNVSSFVY EDSRAFSTEE PLDFEFDENG DIQELTKGTI NSDPSLQAAS QHSNLGSVQR 

       250        260        270        280        290        300 
EYNSEEQESR IHMFEIDEDV LPLPVPLQSV MDSEHNENEP RALKRRKVQK LLEPDENIEL 

       310        320        330        340        350        360 
STRTLSQWRK NYVERMIALE ATKYVRRRGA SSAKKKELNK FFDWESFHPL LKPWIEKLKP 

       370        380        390        400        410        420 
SNNTPSEIDD VLRNIDTSEV EVGRDVQGEL GLNIPWNTSS RSNSAINSKS HSQTGSEHST 

       430        440        450        460        470        480 
PLLDTKYRKR LPHSPSMPSR VEFLPALESS QFHETLNSEL SLQLSDDFVL YKNTQEENAH 

       490        500        510        520        530        540 
LMLSMEKECA NFYEYAKTAI YENNGRITFS SLLPNDLKRP VVAQAFSHLL SLATKSAFLV 

       550        560 
KQDKPYSEIS VSLNLKSTDA I 

References and documentation are available.

Number of amino acids: 561

Molecular weight: 64010.83

Theoretical pI: 5.43

Amino acid composition: 
Ala (A)  28	  5.0%
Arg (R)  26	  4.6%
Asn (N)  36	  6.4%
Asp (D)  32	  5.7%
Cys (C)   2	  0.4%
Gln (Q)  23	  4.1%
Glu (E)  48	  8.6%
Gly (G)  14	  2.5%
His (H)  17	  3.0%
Ile (I)  24	  4.3%
Leu (L)  63	 11.2%
Lys (K)  35	  6.2%
Met (M)  11	  2.0%
Phe (F)  25	  4.5%
Pro (P)  30	  5.3%
Ser (S)  67	 11.9%
Thr (T)  28	  5.0%
Trp (W)   6	  1.1%
Tyr (Y)  15	  2.7%
Val (V)  31	  5.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 80
Total number of positively charged residues (Arg + Lys): 61

Atomic composition:

Carbon      C	      2834
Hydrogen    H	      4437
Nitrogen    N	       773
Oxygen      O	       891
Sulfur      S	        13

Formula: C2834H4437N773O891S13
Total number of atoms: 8948

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    55475
Abs 0.1% (=1 g/l)   0.867, assuming all pairs of Cys residues form cystines


Ext. coefficient    55350
Abs 0.1% (=1 g/l)   0.865, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.70
This classifies the protein as unstable.



Aliphatic index: 81.50

Grand average of hydropathicity (GRAVY): -0.574