ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MFYPYNYSNL NVSTMPALPG ISAFDGMQDE ENVEISPERK YQTLLPVLTN SHVVENELKH 

        70         80         90        100        110        120 
KLNKTAFDFR YQTKSENGSE KWEPKYLITP NLQTRSVSFD NSSVQYNSDS SEKSSLSQLT 

       130        140        150        160        170        180 
CNSSIIQQPE NGIVSNDAYN KMANSRYSLK TRKQRTDPRN TLSDEEDLEQ RRKYICKICA 

       190        200        210        220        230 
RGFTTSGHLA RHNRIHTGEK NHCCPYKGCT QRFSRHDNCL QHYRTHLKKG Q 

References and documentation are available.

Number of amino acids: 231

Molecular weight: 26742.78

Theoretical pI: 8.99

Amino acid composition: 
Ala (A)   7	  3.0%
Arg (R)  15	  6.5%
Asn (N)  20	  8.7%
Asp (D)  10	  4.3%
Cys (C)   7	  3.0%
Gln (Q)  13	  5.6%
Glu (E)  15	  6.5%
Gly (G)   9	  3.9%
His (H)   9	  3.9%
Ile (I)   9	  3.9%
Leu (L)  17	  7.4%
Lys (K)  17	  7.4%
Met (M)   4	  1.7%
Phe (F)   7	  3.0%
Pro (P)  10	  4.3%
Ser (S)  25	 10.8%
Thr (T)  16	  6.9%
Trp (W)   1	  0.4%
Tyr (Y)  12	  5.2%
Val (V)   8	  3.5%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 32

Atomic composition:

Carbon      C	      1153
Hydrogen    H	      1807
Nitrogen    N	       345
Oxygen      O	       368
Sulfur      S	        11

Formula: C1153H1807N345O368S11
Total number of atoms: 3684

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23755
Abs 0.1% (=1 g/l)   0.888, assuming all pairs of Cys residues form cystines


Ext. coefficient    23380
Abs 0.1% (=1 g/l)   0.874, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.45
This classifies the protein as unstable.



Aliphatic index: 56.97

Grand average of hydropathicity (GRAVY): -1.026