ProtParam
User-provided sequence:
10 20 30 40 50 60
MGAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK IIHQDGYSLE
70 80 90 100 110 120
ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA RKLMHMADTI EEGTMPKEMS
130 140 150 160 170 180
DIIQRLWKDS GIQACFDRAS EYQLNDSAGY YLSDLERLVT PGYVPTEQDV LRSRVKTTGI
190 200 210 220 230 240
IETQFSFKDL NFRMFDVGGQ RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH
250 260 270 280 290 300
ESLHLFNSIC NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYDGPN TYEDAGNYIK
310 320 330 340 350
VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF
References and
documentation are available.
Number of amino acids: 350
Molecular weight: 39966.67
Theoretical pI: 5.40
Total number of negatively charged residues (Asp + Glu): 54
Total number of positively charged residues (Arg + Lys): 43
Atomic composition:
Carbon C 1769
Hydrogen H 2791
Nitrogen N 473
Oxygen O 540
Sulfur S 20
Formula: C
1769H
2791N
473O
540S
20
Total number of atoms: 5593
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 30870
Abs 0.1% (=1 g/l) 0.772, assuming all pairs of Cys residues form cystines
Ext. coefficient 30370
Abs 0.1% (=1 g/l) 0.760, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.48
This classifies the protein as stable.
Aliphatic index: 86.94
Grand average of hydropathicity (GRAVY): -0.289