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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGAGASAEEK HSRELEKKLK EDAEKDARTV KLLLLGAGES GKSTIVKQMK IIHQDGYSLE

70 80 90 100 110 120
ECLEFIAIIY GNTLQSILAI VRAMTTLNIQ YGDSARQDDA RKLMHMADTI EEGTMPKEMS

130 140 150 160 170 180
DIIQRLWKDS GIQACFDRAS EYQLNDSAGY YLSDLERLVT PGYVPTEQDV LRSRVKTTGI

190 200 210 220 230 240
IETQFSFKDL NFRMFDVGGQ RSERKKWIHC FEGVTCIIFI AALSAYDMVL VEDDEVNRMH

250 260 270 280 290 300
ESLHLFNSIC NHRYFATTSI VLFLNKKDVF SEKIKKAHLS ICFPDYDGPN TYEDAGNYIK

310 320 330 340 350
VQFLELNMRR DVKEIYSHMT CATDTQNVKF VFDAVTDIII KENLKDCGLF


References and documentation are available.

Number of amino acids: 350 Molecular weight: 39966.67 Theoretical pI: 5.40
Amino acid composition: 
Ala (A) 23 6.6% Arg (R) 17 4.9% Asn (N) 13 3.7% Asp (D) 27 7.7% Cys (C) 8 2.3% Gln (Q) 13 3.7% Glu (E) 27 7.7% Gly (G) 19 5.4% His (H) 9 2.6% Ile (I) 28 8.0% Leu (L) 30 8.6% Lys (K) 26 7.4% Met (M) 12 3.4% Phe (F) 17 4.9% Pro (P) 5 1.4% Ser (S) 22 6.3% Thr (T) 20 5.7% Trp (W) 2 0.6% Tyr (Y) 13 3.7% Val (V) 19 5.4% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 54 Total number of positively charged residues (Arg + Lys): 43 Atomic composition: Carbon C 1769 Hydrogen H 2791 Nitrogen N 473 Oxygen O 540 Sulfur S 20 Formula: C1769H2791N473O540S20 Total number of atoms: 5593 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 30870 Abs 0.1% (=1 g/l) 0.772, assuming all pairs of Cys residues form cystines Ext. coefficient 30370 Abs 0.1% (=1 g/l) 0.760, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 37.48 This classifies the protein as stable. Aliphatic index: 86.94 Grand average of hydropathicity (GRAVY): -0.289