ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGAGTLLNGL EKENFPNNIH SDLPAYPNMD SQEDGNTSKE SKRNSPVKQK SQKDEEKSSK 

        70         80         90        100        110        120 
MGTASNIFHE NKDIHERSEH TDDFNDGLKL APDSSPSLKE CQFKNWESFW CNTEGYKTKH 

       130        140        150        160        170        180 
MQPFHFTSGL EEIKEPVMEL NISTSPYKGQ RPNSAPTEYS AATTAFTKTQ LEVSFLKTNL 

       190        200        210        220        230        240 
LTYIKKEIDI CLSSVPFFDD AVQMQKKFLE YRDIDLDEEY ELKILGELLN DLNFFHMQEN 

       250        260        270        280        290        300 
SLLNRELAVR RFSNQPESQN LPSIRDFRNP LLPIDNRPSP PLGLKRNGKS FEETYDFTSN 

       310        320        330        340        350        360 
TSNFWGEKAE LQNSITGGTP YFFHPNNIHQ TKPFMSFENQ NELLFQRKNS DYKQHFNSGR 

       370        380        390        400        410 
NIHNGVESKS YRGVGLNDSY QKGYAAMTKS FGNIDLNRMP RRSNEEMYSW SRN 

References and documentation are available.

Number of amino acids: 413

Molecular weight: 47699.92

Theoretical pI: 5.85

Amino acid composition: 
Ala (A)  13	  3.1%
Arg (R)  18	  4.4%
Asn (N)  40	  9.7%
Asp (D)  22	  5.3%
Cys (C)   3	  0.7%
Gln (Q)  18	  4.4%
Glu (E)  36	  8.7%
Gly (G)  21	  5.1%
His (H)  11	  2.7%
Ile (I)  16	  3.9%
Leu (L)  34	  8.2%
Lys (K)  32	  7.7%
Met (M)  11	  2.7%
Phe (F)  26	  6.3%
Pro (P)  23	  5.6%
Ser (S)  41	  9.9%
Thr (T)  22	  5.3%
Trp (W)   4	  1.0%
Tyr (Y)  14	  3.4%
Val (V)   8	  1.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 58
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      2099
Hydrogen    H	      3209
Nitrogen    N	       583
Oxygen      O	       665
Sulfur      S	        14

Formula: C2099H3209N583O665S14
Total number of atoms: 6570

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    42985
Abs 0.1% (=1 g/l)   0.901, assuming all pairs of Cys residues form cystines


Ext. coefficient    42860
Abs 0.1% (=1 g/l)   0.899, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 51.46
This classifies the protein as unstable.



Aliphatic index: 55.98

Grand average of hydropathicity (GRAVY): -0.975