ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGAKSVTASS SKKIKNRHNG KVKKSKKIKK VRKPQKSISL NDENEVEILP SRNEQETNKL 

        70         80         90        100        110        120 
PKDHVTADGI LVLEHKSDDD EGFDVYDGHF DNPTDIPSTT EESKTPSLAV HGDEKDLANN 

       130        140        150        160        170        180 
DDFISLSASS EDEQAEQEEE REKQELEIKK EKQKEILNTD YPWILNHDHS KQKEISDWLT 

       190        200        210        220        230        240 
FEIKDFVAYI SPSREEIEIR NQTISTIREA VKQLWPDADL HVFGSYSTDL YLPGSDIDCV 

       250        260        270        280        290        300 
VTSELGGKES RNNLYSLASH LKKKNLATEV EVVAKARVPI IKFVEPHSGI HIDVSFERTN 

       310        320        330        340        350        360 
GIEAAKLIRE WLDDTPGLRE LVLIVKQFLH ARRLNNVHTG GLGGFSIICL VFSFLHMHPR 

       370        380        390        400        410        420 
IITNEIDPKD NLGVLLIEFF ELYGKNFGYD DVALGSSDGY PVYFPKSTWS AIQPIKNPFS 

       430        440        450        460        470        480 
LAIQDPGDES NNISRGSFNI RDIKKAFAGA FDLLTNRCFE LHSATFKDRL GKSILGNVIK 

       490        500        510        520        530        540 
YRGKARDFKD ERGLVLNKAI IENENYHKKR SRIIHDEDFA EDTVTSTATA TTTDDDYEIT 

       550        560        570        580 
NPPAKKAKIE EKPESEPAKR NSGETYITVS SEDDDEDGYN PYTL 

References and documentation are available.

Number of amino acids: 584

Molecular weight: 66030.79

Theoretical pI: 5.31

Amino acid composition: 
Ala (A)  32	  5.5%
Arg (R)  25	  4.3%
Asn (N)  33	  5.7%
Asp (D)  49	  8.4%
Cys (C)   3	  0.5%
Gln (Q)  12	  2.1%
Glu (E)  54	  9.2%
Gly (G)  31	  5.3%
His (H)  18	  3.1%
Ile (I)  46	  7.9%
Leu (L)  45	  7.7%
Lys (K)  54	  9.2%
Met (M)   2	  0.3%
Phe (F)  24	  4.1%
Pro (P)  25	  4.3%
Ser (S)  48	  8.2%
Thr (T)  32	  5.5%
Trp (W)   5	  0.9%
Tyr (Y)  16	  2.7%
Val (V)  30	  5.1%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 103
Total number of positively charged residues (Arg + Lys): 79

Atomic composition:

Carbon      C	      2925
Hydrogen    H	      4599
Nitrogen    N	       799
Oxygen      O	       932
Sulfur      S	         5

Formula: C2925H4599N799O932S5
Total number of atoms: 9260

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    51465
Abs 0.1% (=1 g/l)   0.779, assuming all pairs of Cys residues form cystines


Ext. coefficient    51340
Abs 0.1% (=1 g/l)   0.778, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 41.59
This classifies the protein as unstable.



Aliphatic index: 81.15

Grand average of hydropathicity (GRAVY): -0.680