ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGAKTSKLSK DDLTCLKQST YFDRREIQQW HKGFLRDCPS GQLAREDFVK IYKQFFPFGS 

        70         80         90        100        110        120 
PEDFANHLFT VFDKDNNGFI HFEEFITVLS TTSRGTLEEK LSWAFELYDL NHDGYITFDE 

       130        140        150        160        170        180 
MLTIVASVYK MMGSMVTLNE DEATPEMRVK KIFKLMDKNE DGYITLDEFR EGSKVDPSII 

       190 
GALNLYDGLI 

References and documentation are available.

Number of amino acids: 190

Molecular weight: 22010.98

Theoretical pI: 4.88

Amino acid composition: 
Ala (A)   7	  3.7%
Arg (R)   7	  3.7%
Asn (N)   7	  3.7%
Asp (D)  17	  8.9%
Cys (C)   2	  1.1%
Gln (Q)   5	  2.6%
Glu (E)  15	  7.9%
Gly (G)  12	  6.3%
His (H)   4	  2.1%
Ile (I)  11	  5.8%
Leu (L)  18	  9.5%
Lys (K)  15	  7.9%
Met (M)   7	  3.7%
Phe (F)  16	  8.4%
Pro (P)   5	  2.6%
Ser (S)  12	  6.3%
Thr (T)  13	  6.8%
Trp (W)   2	  1.1%
Tyr (Y)   7	  3.7%
Val (V)   8	  4.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 22

Atomic composition:

Carbon      C	       994
Hydrogen    H	      1514
Nitrogen    N	       248
Oxygen      O	       299
Sulfur      S	         9

Formula: C994H1514N248O299S9
Total number of atoms: 3064

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    21555
Abs 0.1% (=1 g/l)   0.979, assuming all pairs of Cys residues form cystines


Ext. coefficient    21430
Abs 0.1% (=1 g/l)   0.974, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.89
This classifies the protein as stable.



Aliphatic index: 75.42

Grand average of hydropathicity (GRAVY): -0.379