ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGAREDEYDY LFKVVLIGDS GVGKSNLLSR FTRNEFNLES KSTIGVEFAT RSIEVDGKTI 

        70         80         90        100        110        120 
KAQIWDTAGQ ERYRAITSAY YRGAVGALLV YDIAKHLTYE NVERWLRELR DHADQNIVIM 

       130        140        150        160        170        180 
LVGNKSDLRH LRSVPTDEAK LFAERNGLSF IETSALDSTN VETAFQNILT EIYRIVSQKQ 

       190        200        210 
IRDPPEGDVI RPSNVEPIDV KPTVTADVRK QCCQ 

References and documentation are available.

Number of amino acids: 214

Molecular weight: 24245.37

Theoretical pI: 5.53

Amino acid composition: 
Ala (A)  15	  7.0%
Arg (R)  17	  7.9%
Asn (N)  10	  4.7%
Asp (D)  15	  7.0%
Cys (C)   2	  0.9%
Gln (Q)   8	  3.7%
Glu (E)  17	  7.9%
Gly (G)  12	  5.6%
His (H)   3	  1.4%
Ile (I)  16	  7.5%
Leu (L)  17	  7.9%
Lys (K)  11	  5.1%
Met (M)   2	  0.9%
Phe (F)   7	  3.3%
Pro (P)   6	  2.8%
Ser (S)  14	  6.5%
Thr (T)  14	  6.5%
Trp (W)   2	  0.9%
Tyr (Y)   8	  3.7%
Val (V)  18	  8.4%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 32
Total number of positively charged residues (Arg + Lys): 28

Atomic composition:

Carbon      C	      1069
Hydrogen    H	      1706
Nitrogen    N	       302
Oxygen      O	       333
Sulfur      S	         4

Formula: C1069H1706N302O333S4
Total number of atoms: 3414

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    23045
Abs 0.1% (=1 g/l)   0.950, assuming all pairs of Cys residues form cystines


Ext. coefficient    22920
Abs 0.1% (=1 g/l)   0.945, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 36.33
This classifies the protein as stable.



Aliphatic index: 91.54

Grand average of hydropathicity (GRAVY): -0.393