ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGASGRLLRA VIMGAPGSGK GTGSSRITKH FELKHLSSGD LLRQNMLQGT EIAVLAKSFI 

        70         80         90        100        110        120 
DQGKLIPDDD MTRLALHELK NLTQCSWLLD GFPRTLPQAE ALDRVYQIDT VINLNVPFEV 

       130        140        150        160        170        180 
IKLRLTARWI HPASGRVYNI EFNPPKTVGI DDLTGEPLIQ REDDKPETVI KRLKAYEAQT 

       190        200        210        220 
EPVLQYYQKK GVLETFSGTE TNKIRPHVYS FLQMKVPETI QKASVTP 

References and documentation are available.

Number of amino acids: 227

Molecular weight: 25438.34

Theoretical pI: 8.89

Amino acid composition: 
Ala (A)  13	  5.7%
Arg (R)  13	  5.7%
Asn (N)   7	  3.1%
Asp (D)  12	  5.3%
Cys (C)   1	  0.4%
Gln (Q)  12	  5.3%
Glu (E)  14	  6.2%
Gly (G)  16	  7.0%
His (H)   5	  2.2%
Ile (I)  15	  6.6%
Leu (L)  26	 11.5%
Lys (K)  16	  7.0%
Met (M)   5	  2.2%
Phe (F)   7	  3.1%
Pro (P)  14	  6.2%
Ser (S)  12	  5.3%
Thr (T)  17	  7.5%
Trp (W)   2	  0.9%
Tyr (Y)   6	  2.6%
Val (V)  14	  6.2%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	      1138
Hydrogen    H	      1835
Nitrogen    N	       313
Oxygen      O	       334
Sulfur      S	         6

Formula: C1138H1835N313O334S6
Total number of atoms: 3626

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    19940
Abs 0.1% (=1 g/l)   0.784, assuming all pairs of Cys residues form cystines


Ext. coefficient    19940
Abs 0.1% (=1 g/l)   0.784, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.80
This classifies the protein as stable.



Aliphatic index: 94.05

Grand average of hydropathicity (GRAVY): -0.327