ProtParam
User-provided sequence:
10 20 30 40 50 60
MGASGRLLRA VIMGAPGSGK GTGSSRITKH FELKHLSSGD LLRQNMLQGT EIAVLAKSFI
70 80 90 100 110 120
DQGKLIPDDD MTRLALHELK NLTQCSWLLD GFPRTLPQAE ALDRVYQIDT VINLNVPFEV
130 140 150 160 170 180
IKLRLTARWI HPASGRVYNI EFNPPKTVGI DDLTGEPLIQ REDDKPETVI KRLKAYEAQT
190 200 210 220
EPVLQYYQKK GVLETFSGTE TNKIRPHVYS FLQMKVPETI QKASVTP
References and
documentation are available.
Number of amino acids: 227
Molecular weight: 25438.34
Theoretical pI: 8.89
Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 29
Atomic composition:
Carbon C 1138
Hydrogen H 1835
Nitrogen N 313
Oxygen O 334
Sulfur S 6
Formula: C
1138H
1835N
313O
334S
6
Total number of atoms: 3626
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 19940
Abs 0.1% (=1 g/l) 0.784, assuming all pairs of Cys residues form cystines
Ext. coefficient 19940
Abs 0.1% (=1 g/l) 0.784, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.80
This classifies the protein as stable.
Aliphatic index: 94.05
Grand average of hydropathicity (GRAVY): -0.327