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ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGASGRLLRA VIMGAPGSGK GTVSSRITKH FELKHLSSGD LLRQNMLQGT EIGVLAKTFI

70 80 90 100 110 120
DQGKLIPDDV MTRLALHELK NLTQCSWLLD GFPRTLPQAE ALDRVYQIDT VINLNVPFEV

130 140 150 160 170 180
IKLRLTARWI HPASGRVYNI EFNPPKTVGI DDLTGEPLIQ REDDKPETVI KRLKAYEAQT

190 200 210 220
EPVLQYYQKK GVLETFSGTE TNKIWPHVYS FLQMKVPETI QKASVTP


References and documentation are available.

Number of amino acids: 227 Molecular weight: 25494.50 Theoretical pI: 8.89
Amino acid composition: 
Ala (A) 12 5.3% Arg (R) 12 5.3% Asn (N) 7 3.1% Asp (D) 11 4.8% Cys (C) 1 0.4% Gln (Q) 12 5.3% Glu (E) 14 6.2% Gly (G) 16 7.0% His (H) 5 2.2% Ile (I) 15 6.6% Leu (L) 26 11.5% Lys (K) 16 7.0% Met (M) 5 2.2% Phe (F) 7 3.1% Pro (P) 14 6.2% Ser (S) 11 4.8% Thr (T) 18 7.9% Trp (W) 3 1.3% Tyr (Y) 6 2.6% Val (V) 16 7.0% Pyl (O) 0 0.0% Sec (U) 0 0.0% (B) 0 0.0% (Z) 0 0.0% (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 25 Total number of positively charged residues (Arg + Lys): 28 Atomic composition: Carbon C 1147 Hydrogen H 1843 Nitrogen N 311 Oxygen O 332 Sulfur S 6 Formula: C1147H1843N311O332S6 Total number of atoms: 3639 Extinction coefficients: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water. Ext. coefficient 25440 Abs 0.1% (=1 g/l) 0.998, assuming all pairs of Cys residues form cystines Ext. coefficient 25440 Abs 0.1% (=1 g/l) 0.998, assuming all Cys residues are reduced Estimated half-life: The N-terminal of the sequence considered is M (Met). The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro). >20 hours (yeast, in vivo). >10 hours (Escherichia coli, in vivo). Instability index: The instability index (II) is computed to be 32.87 This classifies the protein as stable. Aliphatic index: 96.17 Grand average of hydropathicity (GRAVY): -0.266