ProtParam
User-provided sequence:
10 20 30 40 50 60
MGASGRLLRA VIMGAPGSGK GTVSSRITKH FELKHLSSGD LLRQNMLQGT EIGVLAKTFI
70 80 90 100 110 120
DQGKLIPDDV MTRLALHELK NLTQCSWLLD GFPRTLPQAE ALDRVYQIDT VINLNVPFEV
130 140 150 160 170 180
IKLRLTARWI HPASGRVYNI EFNPPKTVGI DDLTGEPLIQ REDDKPETVI KRLKAYEAQT
190 200 210 220
EPVLQYYQKK GVLETFSGTE TNKIWPHVYS FLQMKVPETI QKASVTP
References and
documentation are available.
Number of amino acids: 227
Molecular weight: 25494.50
Theoretical pI: 8.89
Total number of negatively charged residues (Asp + Glu): 25
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 1147
Hydrogen H 1843
Nitrogen N 311
Oxygen O 332
Sulfur S 6
Formula: C
1147H
1843N
311O
332S
6
Total number of atoms: 3639
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 25440
Abs 0.1% (=1 g/l) 0.998, assuming all pairs of Cys residues form cystines
Ext. coefficient 25440
Abs 0.1% (=1 g/l) 0.998, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.87
This classifies the protein as stable.
Aliphatic index: 96.17
Grand average of hydropathicity (GRAVY): -0.266