ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGASSEQQFC NEQACASSSA NDWGTWSGWS QCSVSCGAGV KRRTRTCRTG NCPGNYKESA 

        70         80         90        100        110        120 
ICNDRDCENK NAAWGGWGYW SSCSETCGDG VRKRVRKCYG SGNCDGQQYE KQYCNLRVCD 


FRRKF 

References and documentation are available.

Number of amino acids: 125

Molecular weight: 13940.32

Theoretical pI: 8.67

Amino acid composition: 
Ala (A)   8	  6.4%
Arg (R)  11	  8.8%
Asn (N)   9	  7.2%
Asp (D)   6	  4.8%
Cys (C)  14	 11.2%
Gln (Q)   7	  5.6%
Glu (E)   6	  4.8%
Gly (G)  15	 12.0%
His (H)   0	  0.0%
Ile (I)   1	  0.8%
Leu (L)   1	  0.8%
Lys (K)   7	  5.6%
Met (M)   1	  0.8%
Phe (F)   3	  2.4%
Pro (P)   1	  0.8%
Ser (S)  14	 11.2%
Thr (T)   5	  4.0%
Trp (W)   6	  4.8%
Tyr (Y)   5	  4.0%
Val (V)   5	  4.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 12
Total number of positively charged residues (Arg + Lys): 18

Atomic composition:

Carbon      C	       576
Hydrogen    H	       875
Nitrogen    N	       187
Oxygen      O	       190
Sulfur      S	        15

Formula: C576H875N187O190S15
Total number of atoms: 1843

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    41325
Abs 0.1% (=1 g/l)   2.964, assuming all pairs of Cys residues form cystines


Ext. coefficient    40450
Abs 0.1% (=1 g/l)   2.902, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 48.89
This classifies the protein as unstable.



Aliphatic index: 24.24

Grand average of hydropathicity (GRAVY): -0.960