ProtParam
User-provided sequence:
10 20 30 40 50 60
MGCASSKHRK RCLHCRRGYS PPVDVQRSHS VHHASQNSED SCHMVALSSS SLGSLKLCDS
70 80 90 100 110 120
SFGHNHKHLA DFSEKLVSGE SVKTGNGFGP NVVREKSDKE KSNLELQAKL MEAKVWSSMM
130 140 150 160 170 180
NEKIPKIVPK TPIVTPPGEP ETINTWEMMD GLEDVLSPLR SPNHVKSFSF DVGPNGGKSN
190 200 210 220 230 240
GSVKPVWLQM EEEEEGFEDF DPEIISSFRK SLQELPSDHP FHISNHDFEL KPRFNFSDEE
250 260 270 280 290 300
KEEEEQSVGK ERVILYFTSL RGIRKTYEES CDVRVILKSL GIRVDERDVS MHSGFKDELK
310 320 330 340 350 360
ELLGEKFNKG VGITLPRVFL GRKYIGGAEE IRKLNEDGKL EKLLGGCERV EENQNGNGLE
370 380 390 400 410 420
CEACGDVRFV PCETCSGSCK VYYEYEDDDD DDDEGDDDES VKEEREYGFQ TCPDCNENGL
IRCPVCCD
References and
documentation are available.
Number of amino acids: 428
Molecular weight: 48178.73
Theoretical pI: 4.98
Total number of negatively charged residues (Asp + Glu): 83
Total number of positively charged residues (Arg + Lys): 55
Atomic composition:
Carbon C 2077
Hydrogen H 3251
Nitrogen N 585
Oxygen O 683
Sulfur S 26
Formula: C
2077H
3251N
585O
683S
26
Total number of atoms: 6622
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 29420
Abs 0.1% (=1 g/l) 0.611, assuming all pairs of Cys residues form cystines
Ext. coefficient 28420
Abs 0.1% (=1 g/l) 0.590, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 47.38
This classifies the protein as unstable.
Aliphatic index: 65.02
Grand average of hydropathicity (GRAVY): -0.748