ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGCASSKHRK RCLHCRRGYS PPVDVQRSHS VHHASQNSED SCHMVALSSS SLGSLKLCDS 

        70         80         90        100        110        120 
SFGHNHKHLA DFSEKLVSGE SVKTGNGFGP NVVREKSDKE KSNLELQAKL MEAKVWSSMM 

       130        140        150        160        170        180 
NEKIPKIVPK TPIVTPPGEP ETINTWEMMD GLEDVLSPLR SPNHVKSFSF DVGPNGGKSN 

       190        200        210        220        230        240 
GSVKPVWLQM EEEEEGFEDF DPEIISSFRK SLQELPSDHP FHISNHDFEL KPRFNFSDEE 

       250        260        270        280        290        300 
KEEEEQSVGK ERVILYFTSL RGIRKTYEES CDVRVILKSL GIRVDERDVS MHSGFKDELK 

       310        320        330        340        350        360 
ELLGEKFNKG VGITLPRVFL GRKYIGGAEE IRKLNEDGKL EKLLGGCERV EENQNGNGLE 

       370        380        390        400        410        420 
CEACGDVRFV PCETCSGSCK VYYEYEDDDD DDDEGDDDES VKEEREYGFQ TCPDCNENGL 


IRCPVCCD 

References and documentation are available.

Number of amino acids: 428

Molecular weight: 48178.73

Theoretical pI: 4.98

Amino acid composition: 
Ala (A)   8	  1.9%
Arg (R)  22	  5.1%
Asn (N)  19	  4.4%
Asp (D)  31	  7.2%
Cys (C)  17	  4.0%
Gln (Q)   8	  1.9%
Glu (E)  52	 12.1%
Gly (G)  35	  8.2%
His (H)  14	  3.3%
Ile (I)  15	  3.5%
Leu (L)  32	  7.5%
Lys (K)  33	  7.7%
Met (M)   9	  2.1%
Phe (F)  18	  4.2%
Pro (P)  21	  4.9%
Ser (S)  43	 10.0%
Thr (T)  10	  2.3%
Trp (W)   3	  0.7%
Tyr (Y)   8	  1.9%
Val (V)  30	  7.0%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 83
Total number of positively charged residues (Arg + Lys): 55

Atomic composition:

Carbon      C	      2077
Hydrogen    H	      3251
Nitrogen    N	       585
Oxygen      O	       683
Sulfur      S	        26

Formula: C2077H3251N585O683S26
Total number of atoms: 6622

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    29420
Abs 0.1% (=1 g/l)   0.611, assuming all pairs of Cys residues form cystines


Ext. coefficient    28420
Abs 0.1% (=1 g/l)   0.590, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 47.38
This classifies the protein as unstable.



Aliphatic index: 65.02

Grand average of hydropathicity (GRAVY): -0.748