ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGCASSKNRN RCRNCKGGLS PVIVPRSYSM HVHHPAQHTG DSYHTVALTS STIGSLSLCD 

        70         80         90        100        110        120 
SSLRHFHKHL EDSFYKQRVS DQMGEETLIS GNGFLHGDEE KMNLDLQAKV IEAKVWSSTI 

       130        140        150        160        170        180 
NEKIPKIVAK TPIVTPPGEP ETINTWELME GLEDVSPLRS PNHLRSFSFD FVRIQPSHDH 

       190        200        210        220        230        240 
DHDVAVSFDL PKSRFHENVK SSCRVDDLDP PDIVSRFKRK TLGKERVVLY FTSLRGIRKT 

       250        260        270        280        290        300 
YEDCCNIRII LKSLGIRIDE RDVSMHSGFK DELKKLLEGK FNNGVGITLP RVFLGNKYLG 

       310        320        330        340        350        360 
GVEEIKKLNE NGELEKLIKD CEMVEDGSPG FGNECEACGD VRFVPCETCS GSCKLYHEGE 

       370        380        390 
EEDEGVTEYG FQRCPYCNEN GLIRCHVCCE 

References and documentation are available.

Number of amino acids: 390

Molecular weight: 43943.76

Theoretical pI: 5.96

Amino acid composition: 
Ala (A)   8	  2.1%
Arg (R)  23	  5.9%
Asn (N)  18	  4.6%
Asp (D)  24	  6.2%
Cys (C)  18	  4.6%
Gln (Q)   6	  1.5%
Glu (E)  36	  9.2%
Gly (G)  31	  7.9%
His (H)  17	  4.4%
Ile (I)  21	  5.4%
Leu (L)  32	  8.2%
Lys (K)  27	  6.9%
Met (M)   7	  1.8%
Phe (F)  16	  4.1%
Pro (P)  18	  4.6%
Ser (S)  34	  8.7%
Thr (T)  16	  4.1%
Trp (W)   2	  0.5%
Tyr (Y)   9	  2.3%
Val (V)  27	  6.9%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 60
Total number of positively charged residues (Arg + Lys): 50

Atomic composition:

Carbon      C	      1911
Hydrogen    H	      3014
Nitrogen    N	       546
Oxygen      O	       594
Sulfur      S	        25

Formula: C1911H3014N546O594S25
Total number of atoms: 6090

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    25535
Abs 0.1% (=1 g/l)   0.581, assuming all pairs of Cys residues form cystines


Ext. coefficient    24410
Abs 0.1% (=1 g/l)   0.555, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 44.72
This classifies the protein as unstable.



Aliphatic index: 75.13

Grand average of hydropathicity (GRAVY): -0.521