ProtParam
User-provided sequence:
10 20 30 40 50 60
MGDAAIRTVV EAIHSSPTQA VVYLCGGASL ALGWLMSVPG ASNTLLESVV PYSRVSMVQL
70 80 90 100 110 120
LGRVPSQHCS QALAKEMALL AYNRALKLSK PGYPVLGVGF TGSLVTSRPK RGDHRFFLSM
130 140 150 160 170 180
RASDRILESS VTLTKNLRSR EEEDKVSSCA LIQAMAKACQ VSGTLDSGLT ESEVPYESET
190 200 210 220 230 240
HFTEEQELEQ LINGHLCCKI YPFSGEAHGS DEDRKIILPG SFNPLHEGHL KLLEVAMSVC
250 260 270 280 290 300
GGGYPCFEIS AINADKPPLT IAQIKDRVKQ FEVVGKTIIV SNQPYFYKKA ELFPGSSFVI
310 320 330 340 350 360
GADTAARLVN PKYYEGSIKR MLEILGDCKR TGCTFLVGGR NVDGVFKVLE DVDIPEEIID
370 380
MFISIPADIF RMDISSTEIR KKQGGGTN
References and
documentation are available.
Number of amino acids: 388
Molecular weight: 42276.62
Theoretical pI: 6.11
Total number of negatively charged residues (Asp + Glu): 45
Total number of positively charged residues (Arg + Lys): 41
Atomic composition:
Carbon C 1871
Hydrogen H 2996
Nitrogen N 506
Oxygen O 566
Sulfur S 20
Formula: C
1871H
2996N
506O
566S
20
Total number of atoms: 5959
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 22515
Abs 0.1% (=1 g/l) 0.533, assuming all pairs of Cys residues form cystines
Ext. coefficient 21890
Abs 0.1% (=1 g/l) 0.518, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 40.67
This classifies the protein as unstable.
Aliphatic index: 91.70
Grand average of hydropathicity (GRAVY): -0.046