ProtParam
User-provided sequence:
10 20 30 40 50 60
MGDAESTKDR LLLPVERVEN VTWSDLRDGS FTVELKRLIF FAAPMAAVVI AQFMLQIVSM
70 80 90 100 110 120
MMVGHLGNLS LASASLASSF CNVTGFSFII GLSCALDTLS GQAYGAKLYR KLGVQTYTAM
130 140 150 160 170 180
FCLALVCLPL SLIWFNMEKL LLILGQDPSI AHEAGKYATW LIPGLFAYAV LQPLTRYFQN
190 200 210 220 230 240
QSLITPLLIT SYVVFCIHVP LCWFLVYNSG LGNLGGALAI SLSNWLYAIF LGSFMYYSSA
250 260 270 280 290 300
CSETRAPLSM EIFDGIGEFF KYALPSAAMI CLEWWSYELI ILLSGLLPNP QLETSVLSVC
310 320 330 340 350 360
LQTISTMYSI PLAIAAAAST RISNELGAGN SRAAHIVVYA AMSLAVIDAL IVSMSLLIGR
370 380 390 400 410 420
NLFGHIFSSD KETIDYVAKM APLVSISLML DALQGVLSGI ARGCGWQHIG AYINLGAFYL
430 440 450 460 470 480
WGIPIAASLA FWIHLKGVGL WIGIQAGAVL QTLLLALVTG CTNWESQADK ARNRMALAYG
T
References and
documentation are available.
Number of amino acids: 481
Molecular weight: 52188.48
Theoretical pI: 6.16
Total number of negatively charged residues (Asp + Glu): 27
Total number of positively charged residues (Arg + Lys): 24
Atomic composition:
Carbon C 2405
Hydrogen H 3753
Nitrogen N 587
Oxygen O 650
Sulfur S 28
Formula: C
2405H
3753N
587O
650S
28
Total number of atoms: 7423
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 94935
Abs 0.1% (=1 g/l) 1.819, assuming all pairs of Cys residues form cystines
Ext. coefficient 94310
Abs 0.1% (=1 g/l) 1.807, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 31.72
This classifies the protein as stable.
Aliphatic index: 119.13
Grand average of hydropathicity (GRAVY): 0.712