ProtParam

User-provided sequence:

        10         20         30         40         50         60 
MGDEPLLQKV KIQEDIESVP LLQKVKIQED IESVKGIRVN NDGEEDGPVT LILLFTTFTA 

        70         80         90        100        110        120 
LCGTFSYGTA AGFTSPAQTG IMAGLNLSLA EFSFFGAVLT IGGLVGAAMS GKLADVFGRR 

       130        140        150        160        170        180 
GALGVSNSFC MAGWLMIAFS QATWSLDIGR LFLGVAAGVA SYVVPVYIVE IAPKKVRGTF 

       190        200        210        220        230        240 
SAINSLVMCA SVAVTYLLGS VISWQKLALI STVPCVFEFV GLFFIPESPR WLSRNGRVKE 

       250        260        270        280        290        300 
SEVSLQRLRG NNTDITKEAA EIKKYMDNLQ EFKEDGFFDL FNPRYSRVVT VGIGLLVLQQ 

       310        320        330        340        350        360 
LGGLSGYTFY LSSIFKKSGF PNNVGVMMAS VVQSVTSVLG IVIVDKYGRR SLLTVATIMM 

       370        380        390        400        410        420 
CLGSLITGLS FLFQSYGLLE HYTPISTFMG VLVFLTSITI GIGGIPWVMI SEMTPINIKG 

       430        440        450        460        470        480 
SAGTLCNLTS WSSNWFVSYT FNFLFQWSSS GVFFIYTMIS GVGILFVMKM VPETRGRSLE 


EIQAAITR 

References and documentation are available.

Number of amino acids: 488

Molecular weight: 53051.02

Theoretical pI: 8.11

Amino acid composition: 
Ala (A)  29	  5.9%
Arg (R)  17	  3.5%
Asn (N)  16	  3.3%
Asp (D)  12	  2.5%
Cys (C)   6	  1.2%
Gln (Q)  15	  3.1%
Glu (E)  22	  4.5%
Gly (G)  49	 10.0%
His (H)   1	  0.2%
Ile (I)  37	  7.6%
Leu (L)  52	 10.7%
Lys (K)  19	  3.9%
Met (M)  17	  3.5%
Phe (F)  34	  7.0%
Pro (P)  15	  3.1%
Ser (S)  47	  9.6%
Thr (T)  32	  6.6%
Trp (W)   8	  1.6%
Tyr (Y)  13	  2.7%
Val (V)  47	  9.6%
Pyl (O)   0	  0.0%
Sec (U)   0	  0.0%

 (B)   0	  0.0%
 (Z)   0	  0.0%
 (X)   0	  0.0%


Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 36

Atomic composition:

Carbon      C	      2431
Hydrogen    H	      3815
Nitrogen    N	       599
Oxygen      O	       680
Sulfur      S	        23

Formula: C2431H3815N599O680S23
Total number of atoms: 7548

Extinction coefficients:

Extinction coefficients are in units of  M-1 cm-1, at 280 nm measured in water.

Ext. coefficient    63745
Abs 0.1% (=1 g/l)   1.202, assuming all pairs of Cys residues form cystines


Ext. coefficient    63370
Abs 0.1% (=1 g/l)   1.195, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.13
This classifies the protein as stable.



Aliphatic index: 105.00

Grand average of hydropathicity (GRAVY): 0.507