ProtParam
User-provided sequence:
10 20 30 40 50 60
MGDEPLLQKV KIQEDIESVP LLQKVKIQED IESVKGIRVN NDGEEDGPVT LILLFTTFTA
70 80 90 100 110 120
LCGTFSYGTA AGFTSPAQTG IMAGLNLSLA EFSFFGAVLT IGGLVGAAMS GKLADVFGRR
130 140 150 160 170 180
GALGVSNSFC MAGWLMIAFS QATWSLDIGR LFLGVAAGVA SYVVPVYIVE IAPKKVRGTF
190 200 210 220 230 240
SAINSLVMCA SVAVTYLLGS VISWQKLALI STVPCVFEFV GLFFIPESPR WLSRNGRVKE
250 260 270 280 290 300
SEVSLQRLRG NNTDITKEAA EIKKYMDNLQ EFKEDGFFDL FNPRYSRVVT VGIGLLVLQQ
310 320 330 340 350 360
LGGLSGYTFY LSSIFKKSGF PNNVGVMMAS VVQSVTSVLG IVIVDKYGRR SLLTVATIMM
370 380 390 400 410 420
CLGSLITGLS FLFQSYGLLE HYTPISTFMG VLVFLTSITI GIGGIPWVMI SEMTPINIKG
430 440 450 460 470 480
SAGTLCNLTS WSSNWFVSYT FNFLFQWSSS GVFFIYTMIS GVGILFVMKM VPETRGRSLE
EIQAAITR
References and
documentation are available.
Number of amino acids: 488
Molecular weight: 53051.02
Theoretical pI: 8.11
Total number of negatively charged residues (Asp + Glu): 34
Total number of positively charged residues (Arg + Lys): 36
Atomic composition:
Carbon C 2431
Hydrogen H 3815
Nitrogen N 599
Oxygen O 680
Sulfur S 23
Formula: C
2431H
3815N
599O
680S
23
Total number of atoms: 7548
Extinction coefficients:
Extinction coefficients are in units of M
-1 cm
-1, at 280 nm measured in water.
Ext. coefficient 63745
Abs 0.1% (=1 g/l) 1.202, assuming all pairs of Cys residues form cystines
Ext. coefficient 63370
Abs 0.1% (=1 g/l) 1.195, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 37.13
This classifies the protein as stable.
Aliphatic index: 105.00
Grand average of hydropathicity (GRAVY): 0.507